Kamzolova S G
Biokhimiia. 1996 Aug;61(8):1483-8.
Activities of RNA polymerases from wild type B/r strain of E. coli and its pleiotropic rpoB403 rif-r mutant were studied using five various DNA matrices and various groups of early region promotors of T4 and T7 phage DNA. The mutation differentially affects RNA polymerase-catalyzed RNA synthesis depending on the promotor used. Relative efficiency of A3 promotor of T7 phage DNA is enhanced in case of the mutant enzyme. rpoB403 mutation can be used for the classification analysis of promotors differing in their interaction with the enzyme.
利用五种不同的DNA模板以及T4和T7噬菌体DNA早期区域启动子的不同组，研究了大肠杆菌野生型B/r菌株及其多效性rpoB403利福平抗性突变体的RNA聚合酶活性。根据所使用的启动子，该突变对RNA聚合酶催化的RNA合成有不同影响。在突变酶的情况下，T7噬菌体DNA的A3启动子的相对效率提高。rpoB403突变可用于对与该酶相互作用不同的启动子进行分类分析。
