Reichert F, Levitzky R, Rotshenker S
Department of Anatomy and Embryology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Eur J Neurosci. 1996 Mar;8(3):530-5. doi: 10.1111/j.1460-9568.1996.tb01237.x.
The multifunctional cytokine interleukin 6 (IL-6) has direct growth, survival and differentiation effects on peripheral and central neurons. Furthermore, it can modulate the production by non-neuronal cells of other cytokines and growth factors, and thereby affect nerve cells indirectly. We have studied IL-6 expression and production in intact and injured peripheral nerves of C57/BL/6NHSD mice, which display the normal rapid progression of Wallerian degeneration. The IL-6 mRNA was detected in nerves degenerating in vitro or in vivo, but not in intact nerves. In vitro- and in vivo-degenerating nerve segments and neuroma nerve segments synthesized and secreted IL-6. The onset of IL-6 production was rapid and prolonged. It was detected as early as 2 h after injury and persisted for the entire period of 21 days tested after the injury. Of the non-neuronal cells that reside in intact and injured nerves, macrophages and fibroblasts were the major contributors to IL-6 production. We also studied IL-6 production in intact and injured nerves of mutant C57BL/6-WLD/OLA/NHSD mice, which display very slow progression of Wallerian degeneration. Injured nerves of C57BL/6-WLD/OLA/NHSD mice produced significantly lower amounts of IL-6 than did rapidly degenerating nerves of C57/BL/6NHSD mice.
多功能细胞因子白细胞介素6（IL-6）对外周和中枢神经元具有直接的生长、存活和分化作用。此外，它可以调节非神经元细胞产生其他细胞因子和生长因子，从而间接影响神经细胞。我们研究了C57/BL/6NHSD小鼠完整和损伤的外周神经中IL-6的表达和产生情况，该小鼠表现出正常的沃勒变性快速进展。在体外或体内退化的神经中检测到了IL-6 mRNA，但在完整神经中未检测到。体外和体内退化的神经节段以及神经瘤神经节段合成并分泌IL-6。IL-6产生的起始迅速且持续时间长。在损伤后2小时就检测到了它，并且在损伤后测试的整个21天期间都持续存在。在完整和损伤神经中存在的非神经元细胞中，巨噬细胞和成纤维细胞是IL-6产生的主要贡献者。我们还研究了突变型C57BL/6-WLD/OLA/NHSD小鼠完整和损伤神经中IL-6的产生情况，该小鼠表现出非常缓慢的沃勒变性进展。C57BL/6-WLD/OLA/NHSD小鼠的损伤神经产生的IL-6量明显低于C57/BL/6NHSD小鼠快速退化的神经。
