Qin P, Pourcho R G
Department of Anatomy and Cell Biology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
Brain Res. 1996 Feb 26;710(1-2):303-7. doi: 10.1016/0006-8993(95)01476-4.
Immunocytochemical techniques were used to localize AMPA-selective glutamate receptor subunits in the cat retina. The antisera employed recognize GluR1, GluR2/3 or GluR4 subunits. Each antiserum produced a distinctive staining pattern which included horizontal cells, cone bipolar cells, and amacrine and ganglion cells. Some cells such as alpha ganglion cells expressed multiple subunits whereas amacrine cells were typically immunoreactive with only one of the antisera.
免疫细胞化学技术被用于在猫视网膜中定位AMPA选择性谷氨酸受体亚基。所使用的抗血清可识别GluR1、GluR2/3或GluR4亚基。每种抗血清都产生了独特的染色模式,包括水平细胞、视锥双极细胞以及无长突细胞和神经节细胞。一些细胞,如α神经节细胞,表达多种亚基,而无长突细胞通常仅对其中一种抗血清产生免疫反应。