Bertini G, Peng Z C, Bentivoglio M
Institute of Anatomy and Histology, University of Verona, Italy.
Brain Res Bull. 1996;39(4):261-6. doi: 10.1016/0361-9230(95)02133-7.
Neurons that contain nitric oxide synthase (NOS) type I and the calcium binding proteins calbindin D28k or parvalbumin were simultaneously visualized by means of double immunohistofluorescence in the cerebral cortex of Wistar and Sprague-Dawley rats. All the three immunoreactive cell populations were primarily represented by nonpyramidal neurons. NOS-immunoreactive cells were less numerous than the calbindin- or parvalbumin-immunoreactive ones, and were intermingled with the neurons containing these calcium binding proteins. NOS-immunoreactive cells were separate from the parvalbumin-immunoreactive ones, whereas a minor proportion of them was found to be colocalized with calbindin. The cortical neurons in which NOS and calbindin coexisted were more numerous in the Sprague-Dawley than in the Wistar rats, and displayed an anteroposterior gradient of density, with the highest concentration in the medial prefrontal, frontal, and cingulate cortices. Double NOS-calbindin-immunoreactive neurons prevailed in the deep cortical layers and they were relatively numerous in the cingulate cortex. The present data indicate a selectivity in the expression of NOS vs. calbindin and parvalbumin in cortical cells, and further support the chemical heterogeneity of GABAergic interneurons in the cerebral cortex.
通过双重免疫荧光法，在Wistar大鼠和Sprague-Dawley大鼠的大脑皮层中同时观察到含有一氧化氮合酶（NOS）I型以及钙结合蛋白钙结合蛋白D28k或小白蛋白的神经元。所有这三种免疫反应性细胞群主要由非锥体神经元组成。NOS免疫反应性细胞比钙结合蛋白或小白蛋白免疫反应性细胞数量少，并与含有这些钙结合蛋白的神经元混合在一起。NOS免疫反应性细胞与小白蛋白免疫反应性细胞分开，而发现其中一小部分与钙结合蛋白共定位。在Sprague-Dawley大鼠中，同时存在NOS和钙结合蛋白的皮层神经元比Wistar大鼠中的更多，并且显示出前后密度梯度，在内侧前额叶、额叶和扣带回皮层中浓度最高。双重NOS-钙结合蛋白免疫反应性神经元在皮层深层占主导地位，并且在扣带回皮层中相对较多。目前的数据表明皮层细胞中NOS与钙结合蛋白和小白蛋白的表达具有选择性，并进一步支持大脑皮层中GABA能中间神经元的化学异质性。
