Coulet M, Eeckhoutte C, Galtier P
Laboratoire de pharmacologie-toxicologie, INRA, Toulouse, France.
Can J Physiol Pharmacol. 1996 Jan;74(1):32-7.
The ontogenic study of the hepatic biotransformation enzymes revealed the early development of both oxidative and conjugative enzymes in male chickens ranging in age from 3 to 12 weeks. Although the rate of microsomal cytochrome P450 reactions progressively increased during the first 9 weeks, it decreased thereafter. Furthermore, the proteins revealed by the antibodies to anti-cytochrome P450 1A2, 2B4, 2C7, and 3A4 appeared to be constitutively expressed. Hepatic monooxygenases were characterized by different developmental patterns. The demethylase activities increased progressively up to 9 weeks, then they declined, in 12 weeks reaching the activity level observed in 3-week-old chickens. In contrast, alkoxyresorufin O-dealkylases and benzopyrene hydroxylase activities continued to increase with age. Significant variability was noted for aniline hydroxylase. Among conjugation enzymes, UDP-glucuronyltransferase towards p-nitrophenol and isoniazid N-acetyltransferase activities increased with the age of the fowl, but with different profiles. Concerning glutathione S-transferase accepting 1-chloro-2,4-dinitrobenzene or 1,2-dichloro-4-nitrobenzene, the chickens aged from 3 to 9 weeks were less well developed in this enzyme than 12-week-old ones.
对雄性鸡肝脏生物转化酶的个体发生学研究表明，3至12周龄的雄性鸡体内氧化酶和结合酶均发育较早。虽然微粒体细胞色素P450反应速率在最初9周内逐渐增加，但此后下降。此外，抗细胞色素P450 1A2、2B4、2C7和3A4抗体所显示的蛋白质似乎是组成性表达的。肝脏单加氧酶具有不同的发育模式。脱甲基酶活性在9周龄前逐渐增加，然后下降，12周龄时达到3周龄鸡所观察到的活性水平。相反，烷氧基试卤灵O-脱烷基酶和苯并芘羟化酶活性随年龄增长持续增加。苯胺羟化酶存在显著变异性。在结合酶中，对硝基苯酚的UDP-葡萄糖醛酸基转移酶和异烟肼N-乙酰转移酶活性随家禽年龄增长而增加，但模式不同。对于接受1-氯-2,4-二硝基苯或1,2-二氯-4-硝基苯的谷胱甘肽S-转移酶，3至9周龄的鸡在该酶方面的发育不如12周龄的鸡。
