Heinze G, King G B, Huerto L, Ortega H, Cortés J, Ariza J R
División de Servicios Clínicos e Investigación, Instituto Mexiano de Pediatría, Calz, México, D.F.
Gac Med Mex. 1994 Jul-Aug;130(4):241-5.
3H-spiperone binding of lymphocytes has been studied and proposed to be a useful vulnerability marker for certain subtypes of schizophrenia. The binding of 3H-spiperone into lymphocytes was studied in 22 schizophrenics, 19 unaffected relatives and 8 healthy subjects. Binding assay was performed, incubating lymphocytes with 0.04 to 0.4 mM of 3H-spiperone. Non-specific binding was measured with 10 mM haloperidol. At the end the lymphocytes were homogenized. The results show a significant (p < 0.01) lower affinity (high Kd values) in drug naive schizophrenics compared with the controls. No differences were found in the binding capacity (Bmax) of both schizophrenic groups compared with control subjects. The results cannot offer us a reliable biological marker for schizophrenia. The increase of the number of subjects is suggested.
已经对淋巴细胞的3H-螺哌隆结合进行了研究，并提出其可作为某些精神分裂症亚型有用的易感性标志物。在22名精神分裂症患者、19名未患病亲属和8名健康受试者中研究了3H-螺哌隆与淋巴细胞的结合。进行结合测定时，将淋巴细胞与0.04至0.4 mM的3H-螺哌隆一起孵育。用10 mM氟哌啶醇测量非特异性结合。最后将淋巴细胞匀浆。结果显示，与对照组相比，未用过药的精神分裂症患者的亲和力显著降低（高Kd值）（p < 0.01）。与对照组相比，两个精神分裂症组的结合能力（Bmax）未发现差异。这些结果无法为我们提供可靠的精神分裂症生物学标志物。建议增加受试者数量。
