Coulter C L, Han V K
MRC Group in Fetal and Neonatal Health and Development, Lawson Research Institute, Ontario, London, Canada.
Horm Res. 1996;45(3-5):167-71. doi: 10.1159/000184781.
The patterns of expression insulin-like growth factor-II (IGF-II) and IGF-binding protein-1 (IGFBP-1) mRNAs were compared between term human and rhesus monkey placenta using in situ hybridization histochemistry. Since IGFs and IGFBPs are paracrine factors, the identification of the sites of synthesis of the IGFs and their binding proteins indicate the potential sites of biological action. In both species, IGF-II mRNA was found in highest abundance in the extravillous cytotrophoblasts. The major difference was observed in placental villi. In the human placenta, IGF-II mRNA was expressed in the chorionic mesoderm of the placental villi, whereas, in the rhesus placenta, it was expressed in the syncytiotrophoblasts and not in the chorionic mesoderm. In both species, IGFBP-1 mRNA was expressed only in the decidua. Therefore, the pattern of expression of IGFBP-1 mRNA in the maternal decidua is similar between rhesus monkey and human placenta, but that of IGF-II mRNA in the fetal placental villi is different. These data suggest that the IGF-II-IGFBP-1 interaction in the paracrine regulation of placental growth and/or function in the rhesus monkey and human placentae may have similarities and differences.
采用原位杂交组织化学方法，比较了足月人类胎盘和恒河猴胎盘胰岛素样生长因子-II（IGF-II）及IGF结合蛋白-1（IGFBP-1）mRNA的表达模式。由于IGF和IGFBP是旁分泌因子，确定IGF及其结合蛋白的合成部位可指示其潜在的生物学作用部位。在这两个物种中，均发现绒毛外细胞滋养层中IGF-II mRNA的丰度最高。胎盘绒毛中观察到主要差异。在人类胎盘中，IGF-II mRNA在胎盘绒毛的绒毛膜中胚层表达，而在恒河猴胎盘中，它在合体滋养层表达，不在绒毛膜中胚层表达。在这两个物种中，IGFBP-1 mRNA仅在蜕膜中表达。因此，恒河猴和人类胎盘母体蜕膜中IGFBP-1 mRNA的表达模式相似，但胎儿胎盘绒毛中IGF-II mRNA的表达模式不同。这些数据表明，恒河猴和人类胎盘中IGF-II - IGFBP-1在胎盘生长和/或功能旁分泌调节中的相互作用可能既有相似之处，也有不同之处。
