将氧-18掺入核苷和碱基中。
Incorporation of oxygen-18 into nucleosides and bases.
作者信息
Puzo G, Schram K H, McCloskey J A
出版信息
Nucleic Acids Res. 1977 Jun;4(6):2075-81. doi: 10.1093/nar/4.6.2075.
Abstract
Extensive incorporation of oxygen-18 at position O4 of the pyrimidine nucleus results from exchange between H218O and nucleosides or bases in 1N HC1 at 100 degrees. The reaction is hindered by substitution at C-5 with the greatest effect shown in pseudouridine (R = ribosy1) and the least in uridine (R = H). Maximum incorporation in the latter compound was 94%, and in uricil was 98%. The method is experimentally simple and the incorporation is readily monitored by mass spectrometry.
摘要
嘧啶核O4位上大量的氧-18掺入是由H218O与1N盐酸中100℃下的核苷或碱基之间的交换引起的。该反应在C-5位被取代所阻碍，假尿苷（R = 核糖基）表现出最大影响，尿苷（R = H）表现出最小影响。后一种化合物的最大掺入率为94%，尿嘧啶的最大掺入率为98%。该方法实验操作简单，掺入情况易于通过质谱监测。
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