锝-99m-肼基烟酰胺免疫球蛋白G的生物分布与剂量测定：与铟-111-二乙三胺五乙酸免疫球蛋白G的比较。

Biodistribution and dosimetry of technetium-99m-hydrazino nicotinamide IgG: comparison with indium-111-DTPA-IgG.

作者信息

Callahan R J, Barrow S A, Abrams M J, Rubin R H, Fischman A J

机构信息

Division of Nuclear Medicine, Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

出版信息

J Nucl Med. 1996 May;37(5):843-6.

PMID:8965158

Abstract

UNLABELLED

The biological behavior of human polyclonal immunoglobulin G (IgG), radiolabeled with 99mTc via a nicotinyl hydrazine derivative (99mTc-HYNIC-IgG), was evaluated in normal human subjects.

METHODS

Initial biodistribution and dosimetry studies were performed in six normal male volunteers. Additionally, 99mTc-IgG and 111In-DTPA-IgG were co-injected into six subjects and scintillation camera images were acquired at 6 and 18 hr later and serial blood and urine samples were collected. Biodistribution of both radiopharmaceuticals were measured by region of interest analysis. In the dual-injection group, images were crossover-corrected.

RESULTS

All subjects tolerated injection of the radiolabeled IgG preparations without apparent ill effects. Biodistribution of the two antibody preparations were remarkably similar with an increase in liver and abdominal activity for the 111In preparation. Linear correlation of the tissue-to-blood ratios of 99mTc and 111In-labeled IgG was observed at both times (r2 > 0.98). The slopes of the regression line were 0.97 and 0.76 at 6 and 18 hr, respectively. The beta phase of the blood clearance of 99mTc-HYNIC-IgG was significantly delayed (p < 0.01) compared with 111In-IgG (t1/2: 51.9 +/- 6.5 versus 35.3 +/- 3.4 hr). In contrast, the volumes of redistribution and urinary excretions of the radiopharmaceuticals were not significantly different.

CONCLUSION

These studies establish that the biodistribution of 99mTc-HYNIC-IgG in normal human subjects is nearly identical to 111In-DTPA-IgG.

摘要

未标记

通过烟酰肼衍生物（99mTc-HYNIC-IgG）用99mTc标记的人多克隆免疫球蛋白G（IgG）的生物学行为在正常人体受试者中进行了评估。

方法

在6名正常男性志愿者中进行了初始生物分布和剂量学研究。此外，将99mTc-IgG和111In-DTPA-IgG共同注射到6名受试者体内，并在6小时和18小时后采集闪烁相机图像，同时收集系列血液和尿液样本。通过感兴趣区分析测量两种放射性药物的生物分布。在双注射组中，对图像进行交叉校正。

结果

所有受试者均耐受放射性标记IgG制剂的注射，无明显不良影响。两种抗体制剂的生物分布非常相似，111In制剂的肝脏和腹部活性增加。在两个时间点均观察到99mTc和111In标记IgG的组织与血液比率呈线性相关（r2>0.98）。回归线的斜率在6小时和18小时分别为0.97和0.76。与111In-IgG相比，99mTc-HYNIC-IgG的血液清除β期明显延迟（p<0.01）（t1/2：51.9±6.5对35.3±3.4小时）。相比之下，放射性药物的再分布体积和尿液排泄量无显著差异。