[Effect of homogenized human pathogenic Helicobacter pylori on the viability of isolated rat gastric mucosal cells].

Aim of this study was to detect the possible direct cellular effect of Helicobacter pylori (Hp) (obtained by biopsy from patients with gastric and duodenal ulcer) on freshly isolated rat gastric mucosal cells. Urease test positive Hp cultured from human biopsy sample was sonicated and incubated with freshly isolated rat gastric mucosal cells. The direct cellular damaging effect of Hp was evaluated alone and in combination with ethanol and indomethacin. The cellular damaging effect was tested morphologically (Trypan blue exclusion) and biochemically (measurements of lactate dehydrogenase, succinic dehydrogenase and ethidium bromide DNA fluorescence). Hp alone, or in combination with ethanol and indomethacin had no direct cellular toxicity on freshly isolated rat gastric mucosal cells. The isolated and cultured Hp collected from human beings did not produce any cellular damage on freshly isolated rat gastric mucosal cells.