Vernazza P L, Weitz M, Siegl G
Institut für Klinische Mikrobiologie und Immunologie, St. Gallen.
Praxis (Bern 1994). 1996 Oct 1;85(40):1245-8.
A microbiological diagnosis is either based on the direct detection of the pathogen or on the reaction of the host (indirect method). Many pathogens, in particular small bacteria and viruses, are undetectable by culture or difficult to cultivate. Molecular methods for the direct detection of such pathogens have been widely introduced in many microbiological laboratories within the past few years. The characteristics of these methods as well as new culture methods and new serological tests are discussed.
微生物诊断要么基于病原体的直接检测,要么基于宿主的反应(间接方法)。许多病原体,特别是小细菌和病毒,无法通过培养检测到或难以培养。在过去几年中,用于直接检测此类病原体的分子方法已在许多微生物实验室中广泛应用。本文将讨论这些方法的特点以及新的培养方法和新的血清学检测方法。