Mio T, Adachi Y, Carnevali S, Romberger D J, Spurzem J R, Rennard S I
Pulmonary and Critical Care Medicine Section, University of Nebraska Medical Center, Omaha 68198-5300, USA.
Am J Physiol. 1996 May;270(5 Pt 1):L829-35. doi: 10.1152/ajplung.1996.270.5.L829.
The effects of beta-adrenergic agonists on fibroblast-mediated collagen gel contraction were investigated. beta-Agonists isoproterenol and epinephrine significantly attenuated fibroblast-mediated gel contraction in a concentration-dependent manner, whereas alpha-agonist norepinephrine had no effect. The biologically active form of isoproterenol, (-)-isoproterenol, was 10-fold more effective than the optical isoform, (+)-isoproterenol. beta-Antagonists sotalol and propranolol reversed the attenuation caused by 10(-7) M isoproterenol or epinephrine at the concentration of 10(-7) M or 10(-6) M, but the alpha-antagonist phentolamine did not. However, beta1- or beta2-specificity of these effects is not clear. Isobutyl methylxanthine augmented the effect of isoproterenol and also prolonged the duration. Two reagents which are known to increase intracellular adenosine 3',5'-cyclic monophosphate (cAMP), prostaglandin E2 and dibutyryl adenosine 3',5'-cyclic monophosphate, attenuated gel contraction in a concentration-dependent manner. These data suggest that the fibroblast-mediated collagen gel contraction can be modulated by beta-adrenergic agonists and that the effect depends on cAMP.
研究了β-肾上腺素能激动剂对成纤维细胞介导的胶原凝胶收缩的影响。β-激动剂异丙肾上腺素和肾上腺素以浓度依赖的方式显著减弱成纤维细胞介导的凝胶收缩，而α-激动剂去甲肾上腺素则无作用。异丙肾上腺素的生物活性形式（-）-异丙肾上腺素比光学异构体（+）-异丙肾上腺素的效力高10倍。β-拮抗剂索他洛尔和普萘洛尔在10^-7 M或10^-6 M的浓度下可逆转由10^-7 M异丙肾上腺素或肾上腺素引起的减弱作用，但α-拮抗剂酚妥拉明则不能。然而，这些作用的β1或β2特异性尚不清楚。异丁基甲基黄嘌呤增强了异丙肾上腺素的作用并延长了作用持续时间。已知的两种可增加细胞内3',5'-环磷酸腺苷（cAMP）的试剂，前列腺素E2和二丁酰3',5'-环磷酸腺苷，以浓度依赖的方式减弱凝胶收缩。这些数据表明，成纤维细胞介导的胶原凝胶收缩可被β-肾上腺素能激动剂调节，且该作用依赖于cAMP。
