被寄生的粘虫幼虫中多角体病毒编码mRNA的特性分析

Characterization of polydnavirus-encoded mRNA in parasitized armyworm larvae.

作者信息

Yamanaka A, Hayakawa Y, Noda H, Nakashima N, Watanabe H

出版信息

Insect Biochem Mol Biol. 1996 Jun;26(6):529-36. doi: 10.1016/0965-1748(95)00107-7.

Abstract

We have isolated five cDNA clones encoding Cotesia kariyai polydnavirus (CkPDV) mRNAs transcribed in parasitized host larvae of Pseudaletia separata. One of the cDNAs encoding the longest 2.0 kb CkPDV mRNA was sequenced and characterized. Southern hybridization analyses using the cloned cDNA as a probe showed that CkPDV mRNA was homologous to one of CkPDV DNA segments, 5.6 kbp DNA segment A. The 2.0-kb mRNA was demonstrated as being expressed in the parasitized host larvae by Northern-blot analyses. When specific host tissues were examined, the 2.0-kb mRNA was detected mainly in haemocytes. This RNA increased in relative abundance after 2 and 4 h post-parasitization when the immune response of host haemocytes appeared compromised.

摘要

我们从被分离夜蛾（Pseudaletia separata）寄生的宿主幼虫中转录的编码甘蓝夜蛾盘绒茧蜂多DNA病毒（Cotesia kariyai polydnavirus，CkPDV）mRNA中分离出了五个cDNA克隆。对其中一个编码最长2.0 kb CkPDV mRNA的cDNA进行了测序和表征。使用克隆的cDNA作为探针进行的Southern杂交分析表明，CkPDV mRNA与CkPDV DNA片段之一、5.6 kbp的DNA片段A同源。通过Northern印迹分析证明，2.0 kb的mRNA在被寄生的宿主幼虫中表达。当检查特定的宿主组织时，主要在血细胞中检测到2.0 kb的mRNA。在寄生后2小时和4小时，当宿主血细胞的免疫反应似乎受到损害时，这种RNA的相对丰度增加。