Miller J J, Bieber M M, Levinson J E, Zhu S, Tsou E, Teng N N
Department of Pediatrics, Stanford University School of Medicine, California, USA.
J Rheumatol. 1996 Dec;23(12):2132-9.
To determine if the germ line gene VH4-34 (VH4.21) encodes the antimonophosphoryl lipid A (MPL) polyspecific antibodies found in oligoarticular arthritis of childhood.
Sera from a range of rheumatic diseases of childhood were assayed for VH4-34 derived antibodies by ELISA using the antiidiotype monoclonal antibody 9G4. Results were compared to assays for anti-MPL antibodies, C4d, and Bb, and for HLA type, joint count, and sedimentation rate.
VH4-34 derived antibodies were elevated in all diseases studied except rheumatoid factor positive polyarticular disease. In oligoarticular arthritis, VH4-34 gene expression correlated with C4d concentration, and VH4-34 encoded globulins were more concentrated in synovial fluid than in blood. No association was found with HLA type. An association between VH4-34 expression and IgG anti-MPL was found in sera from patients from Cincinnati but not from Stanford. No other evidence supported a direct association between VH4-34 derived and anti-MPL antibodies in these children.
The expression of VH4-34 is increased in several rheumatic diseases of childhood, but, as in adults, not in rheumatoid arthritis. VH4-34 expression is not associated with HLA type. The polyspecific autoantibody nature of some VH4-34 derived antibodies may explain the wide range of the unusual antibodies found in oligoarticular arthritis.
确定种系基因VH4 - 34（VH4.21）是否编码在儿童寡关节炎中发现的抗单磷酸脂A（MPL）多特异性抗体。
使用抗独特型单克隆抗体9G4通过ELISA检测一系列儿童风湿性疾病血清中VH4 - 34衍生抗体。将结果与抗MPL抗体、C4d、Bb检测结果以及HLA类型、关节计数和血沉率检测结果进行比较。
除类风湿因子阳性多关节炎疾病外，在所有研究的疾病中VH4 - 34衍生抗体均升高。在寡关节炎中，VH4 - 34基因表达与C4d浓度相关，且VH4 - 34编码的球蛋白在滑液中比在血液中更浓缩。未发现与HLA类型有关联。在辛辛那提患者的血清中发现VH4 - 34表达与IgG抗MPL之间存在关联，但斯坦福患者的血清中未发现。没有其他证据支持这些儿童中VH4 - 34衍生抗体与抗MPL抗体之间存在直接关联。
VH4 - 34的表达在几种儿童风湿性疾病中增加，但与成人一样，在类风湿关节炎中不增加。VH4 - 34表达与HLA类型无关。一些VH4 - 34衍生抗体的多特异性自身抗体性质可能解释了在寡关节炎中发现的广泛的异常抗体。
