Wang Y, Ichiba M, Oishi H, Iyadomi M, Shono N, Tomokuni K
Department of Community Health Science, Saga Medical School, Japan.
Nutr Cancer. 1997;27(1):69-73. doi: 10.1080/01635589709514504.
beta-Carotene and alpha-tocopherol have been thought to reduce risk of lung cancer. Whether beta-carotene and alpha-tocopherol influence human DNA adducts, indicators of biologically effective doses of carcinogens, has been seldom studied. In this cross-sectional study, we measured plasma beta-carotene and alpha-tocopherol in 192 healthy men and DNA adducts in lymphocytes in 104 of the subjects. Because genetic polymorphism of cytochrome P-4501A1 (CYP1A1) and glutathione S-transferase M1 (GSTM1) has been associated with interference in formation of reactive intermediates and detoxification of polycyclic aromatic hydrocarbons, we also obtained data concerning genetic polymorphism of CYP1A1 and GSTM1. In multiple regression analysis, parameters such as alcohol consumed per day, high-density lipoprotein cholesterol, Quetelet index, and cigarettes smoked per day were correlated inversely, whereas age, plasma alpha-tocopherol, and intake frequency of fruits were correlated positively with plasma beta-carotene concentration. DNA adduct levels of high plasma beta-carotene or alpha-tocopherol groups were not significantly different from the DNA adduct levels of low plasma beta-carotene or alpha-tocopherol groups among current smokers or nonsmokers. In variant states of CYP1A1 or GSTM1 polymorphism, after controlling for effect of cigarettes smoked per day, no significant correlation was found between plasma beta-carotene or alpha-tocopherol and DNA adduct levels. These results indicated that alcohol consumption, cigarette smoking, and plasma alpha-tocopherol have a close relationship with plasma beta-carotene. The plasma beta-carotene and alpha-tocopherol were not likely to influence the level of DNA adducts in lymphocytes.
β-胡萝卜素和α-生育酚被认为可降低肺癌风险。β-胡萝卜素和α-生育酚是否会影响人类DNA加合物（致癌物生物有效剂量的指标），这方面的研究很少。在这项横断面研究中，我们测量了192名健康男性的血浆β-胡萝卜素和α-生育酚，并测量了其中104名受试者淋巴细胞中的DNA加合物。由于细胞色素P-4501A1（CYP1A1）和谷胱甘肽S-转移酶M1（GSTM1）的基因多态性与活性中间体形成的干扰以及多环芳烃的解毒有关，我们还获取了有关CYP1A1和GSTM1基因多态性的数据。在多元回归分析中，每天饮酒量、高密度脂蛋白胆固醇、体重指数和每天吸烟量等参数呈负相关，而年龄、血浆α-生育酚和水果摄入频率与血浆β-胡萝卜素浓度呈正相关。当前吸烟者或非吸烟者中，高血浆β-胡萝卜素或α-生育酚组的DNA加合物水平与低血浆β-胡萝卜素或α-生育酚组的DNA加合物水平无显著差异。在CYP1A1或GSTM1基因多态性的变异状态下，在控制了每天吸烟的影响后，血浆β-胡萝卜素或α-生育酚与DNA加合物水平之间未发现显著相关性。这些结果表明，饮酒、吸烟和血浆α-生育酚与血浆β-胡萝卜素密切相关。血浆β-胡萝卜素和α-生育酚不太可能影响淋巴细胞中DNA加合物的水平。
