Chan F K, Zhang Y, Shaffer E A
Department of Medicine, Faculty of Medicine, University of Calgary, Alberta, Canada.
Transplantation. 1996 Dec 15;62(11):1657-63. doi: 10.1097/00007890-199612150-00021.
Arterialized and nonarterialized rat models of orthotopic liver transplantation are widely used for studying various aspects of graft function. Although bile duct damage has been implicated in graft nonarterialization, any impact on graft secretory function is unknown. This study sought to investigate whether nonarterialized orthotopic liver transplantation (NOLT) is a valid model for studying bile formation after the animal has fully recovered from the perioperative period. Twenty-four-hour bile collections were performed on eight arterialized and eight nonarterialized rats 4 weeks after transplantation to avoid the confounding effects of preservation-reperfusion injury and perioperative stress. Eight unoperated rats were used as control. There was no difference in mortality or biliary complications between the two transplant groups. The nonarterialized rats exhibited a higher serum aminotransferase level, but serum bilirubin was normal. NOLT resulted in more portal lymphocytic infiltration and bile ductular proliferation. Despite these histologic changes, bile duct epithelial cells remained intact, and spontaneous graft rearterialization was evident in the NOLT group. Bile salt secretion, pool size, and synthesis in both transplant groups did not differ from unoperated rats. NOLT did not adversely affect either bile acid-dependent or bile acid-independent flow. Biliary cholesterol secretion was markedly reduced in both transplant groups, resulting in a more favorable cholesterol saturation index. In conclusion, hepatic allograft secretory function is well maintained at 4 weeks even in the absence of hepatic arterial reanastomosis. Compensatory mechanisms possibly prevent irreversible hepatobiliary damage in NOLT. The NOLT model is quite reasonable to study bile formation after transplantation.
原位肝移植的动脉化和非动脉化大鼠模型被广泛用于研究移植物功能的各个方面。尽管胆管损伤与移植物非动脉化有关，但对移植物分泌功能的任何影响尚不清楚。本研究旨在调查非动脉化原位肝移植（NOLT）在动物从围手术期完全恢复后是否是研究胆汁形成的有效模型。移植后4周对8只动脉化大鼠和8只非动脉化大鼠进行24小时胆汁收集，以避免保存-再灌注损伤和围手术期应激的混杂影响。8只未手术的大鼠用作对照。两个移植组在死亡率或胆道并发症方面没有差异。非动脉化大鼠的血清转氨酶水平较高，但血清胆红素正常。NOLT导致更多的门静脉淋巴细胞浸润和胆小管增生。尽管有这些组织学变化，但胆管上皮细胞保持完整，并且在NOLT组中自发的移植物再动脉化明显。两个移植组的胆汁盐分泌、池大小和合成与未手术大鼠没有差异。NOLT对胆汁酸依赖性或胆汁酸非依赖性胆汁流动均无不利影响。两个移植组的胆汁胆固醇分泌均明显减少，导致胆固醇饱和指数更有利。总之，即使在没有肝动脉重新吻合的情况下，肝同种异体移植的分泌功能在4周时仍能得到良好维持。代偿机制可能会防止NOLT中不可逆的肝胆损伤。NOLT模型对于研究移植后胆汁形成是相当合理的。
