托高托病毒（正粘病毒科）mRNA的5'末端在长度和序列上均具有一致性。
The 5' ends of Thogoto virus (Orthomyxoviridae) mRNAs are homogeneous in both length and sequence.
作者信息
Albo C, Martín J, Portela A
机构信息
Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Madrid, Spain.
出版信息
J Virol. 1996 Dec;70(12):9013-7. doi: 10.1128/JVI.70.12.9013-9017.1996.
Abstract
Thogoto (THO) virus is a tick-borne member of the Orthomyxoviridae whose genome consists of six segments of linear, negative sense, single-stranded RNA. To gain insight into the mechanism by which viral mRNA transcripts are initiated, poly(A)+ RNA isolated from THO virus-infected cells was characterized by (i) primer extension experiments, (ii) immunoprecipitation studies with an anticap monoclonal antibody, (iii) direct sequencing analysis of the isolated RNA, and (iv) cloning and sequencing of individual mRNA molecules. The results indicated that THO virus mRNAs are capped and homogeneous in both length and sequence at their 5' end. These findings contrast with the situation found in all other segmented, negative sense or ambisense, single-stranded RNA viruses so far analyzed in which the 5' ends of viral mRNAs are heterogeneous in length and sequence. These results are discussed in terms of the mechanism used by THO virus to initiate mRNA synthesis.
摘要
托戈托（THO）病毒是正粘病毒科的一种蜱传病毒，其基因组由6个线性、负义、单链RNA片段组成。为深入了解病毒mRNA转录本起始的机制，从感染THO病毒的细胞中分离出的多聚腺苷酸（poly(A)）+RNA通过以下方法进行了表征：（i）引物延伸实验；（ii）用抗帽单克隆抗体进行免疫沉淀研究；（iii）对分离出的RNA进行直接测序分析；（iv）对单个mRNA分子进行克隆和测序。结果表明，THO病毒mRNA在其5'端被加帽，长度和序列均一。这些发现与迄今为止分析的所有其他分段的、负义或双义的单链RNA病毒的情况形成对比，在这些病毒中，病毒mRNA的5'端在长度和序列上是异质的。根据THO病毒启动mRNA合成所使用的机制对这些结果进行了讨论。
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