猫泡沫病毒剪接后的pol转录本的特征：pol转录本的剪接受体位点位于泡沫病毒的gag基因中。
Characterization of the spliced pol transcript of feline foamy virus: the splice acceptor site of the pol transcript is located in gag of foamy viruses.
作者信息
Bodem J, Löchelt M, Winkler I, Flower R P, Delius H, Flügel R M
机构信息
Abteilung Retrovirale Genexpression, Forschungsschwerpunkt Angewandte Tumorvirologie, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
出版信息
J Virol. 1996 Dec;70(12):9024-7. doi: 10.1128/JVI.70.12.9024-9027.1996.
Abstract
Foamy viruses, or spumaviruses, are distinct members of the Retroviridae. Here we have characterized the long terminal repeat of the feline, or cat, foamy virus by determining the locations of the transcriptional start site and the poly(A) addition site. The splice donor and splice acceptor sites of the subgenomic mRNA responsible for Pro-Pol protein expression were identified by nucleotide sequencing of the corresponding cDNAs. The leader exon of the feline foamy virus is 57 nucleotides long. The splice acceptor of the subgenomic pol mRNA was found to be located in gag. The location of the splice acceptor of the human foamy virus pol mRNA was confirmed to map in gag. The pol splice acceptor site in gag of the cat foamy virus is located further downstream than that of human foamy virus.
摘要
泡沫病毒，即泡沫逆转录病毒，是逆转录病毒科中独特的成员。在此，我们通过确定转录起始位点和多聚腺苷酸（poly(A)）添加位点的位置，对猫泡沫病毒的长末端重复序列进行了特征分析。通过对相应cDNA进行核苷酸测序，确定了负责Pro-Pol蛋白表达的亚基因组mRNA的剪接供体和剪接受体位点。猫泡沫病毒的前导外显子长度为57个核苷酸。发现亚基因组pol mRNA的剪接受体位点位于gag基因中。人泡沫病毒pol mRNA的剪接受体位点经确认也定位于gag基因中。猫泡沫病毒gag基因中的pol剪接受体位点比人泡沫病毒的该位点更靠下游。
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Foamy virus reverse transcriptase is expressed independently from the Gag protein.泡沫病毒逆转录酶独立于 gag 蛋白表达。
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