Wang T K, Fuh M S
Department of Chemistry, Soochow University, Taipei, Taiwan.
J Chromatogr B Biomed Appl. 1996 Nov 15;686(2):285-90. doi: 10.1016/s0378-4347(96)00228-9.
A simple procedure for the determination of amphetamine in urine with minimal sample preparation is described. This method involves direct addition of human urine to an acetone-dansyl chloride solution for simultaneous deproteinization and fluorescence derivatization. The derivatized amphetamine is then measured by HPLC with fluorescence detection. It eliminates the extraction procedures often required by other HPLC or GC methods. The effects of pH, temperature and reaction time on the derivatization reaction were investigated. The stability of amphetamine-dansyl chloride in different storage conditions was examined. The detection limit and linearity associated with this assay are discussed.
描述了一种用于测定尿液中苯丙胺的简单程序，样品前处理最少。该方法包括将人尿液直接加入丙酮 - 丹磺酰氯溶液中，以同时进行蛋白质沉淀和荧光衍生化。然后通过高效液相色谱 - 荧光检测法测定衍生化的苯丙胺。它省去了其他高效液相色谱或气相色谱方法通常需要的萃取步骤。研究了pH值、温度和反应时间对衍生化反应的影响。考察了丹磺酰氯 - 苯丙胺在不同储存条件下的稳定性。讨论了该测定方法的检测限和线性关系。
