Frye R F, Stiff D D
Department of Pharmacy and Therapeutics, School of Pharmacy, University of Pittsburgh Medical Center, University of Pittsburgh, PA 15261, USA.
J Chromatogr B Biomed Appl. 1996 Nov 15;686(2):291-6. doi: 10.1016/s0378-4347(96)00227-7.
A sensitive and reproducible method is described for the determination of the cytochrome P450 enzyme 2E1 substrate chlorzoxazone and its primary metabolite 6-hydroxychlorzoxazone in human plasma and urine. Plasma or diluted urine were acidified, incubated with beta-glucuronidase and then were extracted with diethyl ether. Separation of the analytes was achieved on a C18 column with UV detection set at 283 nm. Excellent linearity was observed over the concentration ranges of 100-3000 ng/ml and 4-400 micrograms/ml in plasma and urine, respectively. The intra-assay variability was < or = 5.1% and the inter-assay variability was < or = 8.2% for each compound in each matrix. The method presented is applicable to pharmacokinetic and pharmacogenetic studies utilizing chlorzoxazone.
本文描述了一种灵敏且可重复的方法，用于测定人血浆和尿液中细胞色素P450酶2E1的底物氯唑沙宗及其主要代谢物6-羟基氯唑沙宗。将血浆或稀释后的尿液酸化，与β-葡萄糖醛酸酶一起孵育，然后用乙醚萃取。在C18柱上实现了分析物的分离，紫外检测波长设定为283nm。在血浆和尿液中，氯唑沙宗的浓度范围分别为100-3000ng/ml和4-400μg/ml时，均观察到良好的线性关系。每种基质中每种化合物的批内变异系数≤5.1%，批间变异系数≤8.2%。所提出的方法适用于利用氯唑沙宗进行的药代动力学和药物遗传学研究。
