Rajyalakshmi T, Srinivas T, Swamy K V, Prasad N S, Mohan P M
Department of Zoology, Sri Venkateswara University, Tirupati, India.
Drug Chem Toxicol. 1996 Nov;19(4):325-31. doi: 10.3109/01480549608998241.
Butachlor action on acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) activates in central nervous tissue of the snail Pila globosa was assayed following the method of ELLMAN et al1, in vitro by adding butachlor directly (10-100 mu moles), to tissue homogenates and in in vivo by exposing the snails to sub-lethal concentration (26.6 ppm) and taking out the tissue for experimentation at different intervals (3, 6, 12, 24 and 48 h) of exposure. The enzyme activities decreased in a dose-dependent manner in vitro, and up to 12-24 h in vivo after which they showed recovery towards the control. The inhibition of cholinesterases by butachlor in vitro indicates a direct action of the herbicide on these enzymes. Presumably butachlor exercises its neurotoxic effects through cholinergic impairment in a way similar to that of organophosphates and carbamates.
按照ELLMAN等人[1]的方法，通过直接向组织匀浆中添加丁草胺（10 - 100微摩尔）在体外测定丁草胺对福寿螺中枢神经组织中乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BuChE）的激活作用，并通过将福寿螺暴露于亚致死浓度（26.6 ppm）并在暴露的不同时间间隔（3、6、12、24和48小时）取出组织进行体内实验。在体外，酶活性呈剂量依赖性降低，在体内直至12 - 24小时后酶活性恢复至对照水平。丁草胺在体外对胆碱酯酶的抑制表明该除草剂对这些酶有直接作用。推测丁草胺通过与有机磷酸酯和氨基甲酸酯类似的方式通过胆碱能损伤发挥其神经毒性作用。
