使用针对还原糖化赖氨酸的单克隆抗体，对非胰岛素依赖型糖尿病患者的糖尿病肾病肾活检样本进行免疫荧光染色。

Immunofluorescence staining of renal biopsy samples in patients with diabetic nephropathy in non-insulin-dependent diabetes mellitus using monoclonal antibody to reduced glycated lysine.

作者信息

Suzuki D, Yagame M, Jinde K, Naka R, Yano N, Endoh M, Kaneshige H, Nomoto Y, Sakai H

机构信息

Department of Internal Medicine, School of Medicine, Tokai University, Kanagawa, Japan.

出版信息

J Diabetes Complications. 1996 Nov-Dec;10(6):314-9. doi: 10.1016/1056-8727(95)00060-7.

DOI:10.1016/1056-8727(95)00060-7

PMID:8972382

Abstract

This is the first report on immunofluorescence staining of renal biopsy samples in human diabetic nephropathy (DN) using monoclonal antibodies to reduced glycated lysine. In order to detect the localization of glycated lysine in the mesangial matrix and/or the glomerular basement membrane (GBM), we examined immunofluorescence staining using antibodies against reduced glycated lysine in the glomeruli of 16 patients with DN and ten age-matched patients with diffuse mesangial proliferative glomerulonephritis without IgA deposition (DPGN) as controls. In the early stage of DN, immunofluorescence microscopy revealed the presence of intense staining for reduced glycated lysine in the GBM as well as in part of the tubular basement membrane, but not in the mesangial area. In contrast, immunofluorescence microscopy revealed less staining for glycated lysine in the GBM in the advanced stage of DN, and no reaction with any part of the renal tissue in patients with DPGN. It was concluded that detection of reduced glycated lysine in GBM in the early stage of DN might be associated with the initial pathogenesis of this disease.

摘要

这是关于使用抗还原糖化赖氨酸单克隆抗体对人类糖尿病肾病（DN）肾活检样本进行免疫荧光染色的首篇报告。为检测糖化赖氨酸在系膜基质和/或肾小球基底膜（GBM）中的定位，我们使用抗还原糖化赖氨酸抗体对16例DN患者以及10例年龄匹配的无IgA沉积的弥漫性系膜增生性肾小球肾炎（DPGN）患者的肾小球进行免疫荧光染色检查，后者作为对照。在DN早期，免疫荧光显微镜检查显示GBM以及部分肾小管基底膜中有强烈的还原糖化赖氨酸染色，但系膜区无染色。相比之下，免疫荧光显微镜检查显示DN晚期GBM中糖化赖氨酸染色较少，而DPGN患者的肾组织任何部位均无反应。得出的结论是，DN早期GBM中还原糖化赖氨酸的检测可能与该疾病的初始发病机制有关。