Coutts R H, Coffin R S
Biology Department, Imperial College of Science, Technology and Medicine, London, UK.
Virus Genes. 1996;13(2):179-81. doi: 10.1007/BF00568911.
We have previously used RT-PCR to amplify a 251 base pair fragment diagnostic for beet pseudo-yellows virus (BPYV) infection from several BPYV infected samples from Europe and the USA. This has now been shown by sequencing to represent a fragment of a heat shock protein (HSP)70 gene. Sequence analysis of the fragment revealed that it is closely similar to the C-terminal sequence of the HSP70 homologue gene of lettuce infectious yellows virus, a definitive closterovirus, and also has obvious sequence similarities to the HSP70 genes of the other previously sequenced closteroviruses citrus tristeza virus and beet yellows virus. This observation confirms that BPYV is an authentic closterovirus with a wide geographical distribution.
我们之前使用逆转录聚合酶链反应（RT-PCR）从欧洲和美国的多个感染甜菜假黄化病毒（BPYV）的样本中扩增出一段251个碱基对的片段，该片段可用于诊断BPYV感染。现在通过测序表明，这段片段代表热休克蛋白（HSP）70基因的一个片段。对该片段的序列分析显示，它与莴苣传染性黄化病毒（一种典型的长线形病毒）的HSP70同源基因的C末端序列非常相似，并且与其他先前测序的长线形病毒柑橘衰退病毒和甜菜黄化病毒的HSP70基因也有明显的序列相似性。这一观察结果证实BPYV是一种真正的长线形病毒，具有广泛的地理分布。
