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本文引用的文献

1
PF1: an A-T hook-containing DNA binding protein from rice that interacts with a functionally defined d(AT)-rich element in the oat phytochrome A3 gene promoter.PF1：一种来自水稻的含A - T钩的DNA结合蛋白，它与燕麦光敏色素A3基因启动子中一个功能明确的富含d(AT)的元件相互作用。
Plant Cell. 1994 Feb;6(2):287-301. doi: 10.1105/tpc.6.2.287.
2
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.GUS融合：β-葡萄糖醛酸酶作为高等植物中一种灵敏且通用的基因融合标记
EMBO J. 1987 Dec 20;6(13):3901-7. doi: 10.1002/j.1460-2075.1987.tb02730.x.
3
Efficient transformation of Agrobacterium spp. by high voltage electroporation.通过高压电穿孔实现农杆菌属的高效转化。
Nucleic Acids Res. 1989 Oct 25;17(20):8385. doi: 10.1093/nar/17.20.8385.
4
A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene.一个与S位点特异性糖蛋白结构基因具有同源性的高度保守的芸苔属基因。
Plant Cell. 1989 Feb;1(2):249-58. doi: 10.1105/tpc.1.2.249.
5
Genomic sequence of a Brassica S locus-related gene.
Plant Mol Biol. 1990 Jul;15(1):203-5. doi: 10.1007/BF00017746.
6
A Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana.一个芸苔属S位点基因启动子将毒性基因表达和细胞死亡靶向到转基因烟草的雌蕊和花粉。
Dev Biol. 1991 Jan;143(1):173-84. doi: 10.1016/0012-1606(91)90064-a.
7
Structure and expression of AtS1, an Arabidopsis thaliana gene homologous to the S-locus related genes of Brassica.拟南芥中与芸苔属S位点相关基因同源的AtS1基因的结构与表达
Mol Gen Genet. 1992 Feb;231(3):442-8. doi: 10.1007/BF00292714.

甘蓝型油菜SLR1基因启动子的功能分析

Functional analysis of a Brassica oleracea SLR1 gene promoter.

作者信息

Hackett R M, Cadwallader G, Franklin F C

机构信息

School of Biological Sciences, University of Birmingham, Edgbaston, United Kingdom.

出版信息

Plant Physiol. 1996 Dec;112(4):1601-7. doi: 10.1104/pp.112.4.1601.

DOI:10.1104/pp.112.4.1601
PMID:8972601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC158093/
Abstract

The Brassica oleracea S-locus-related gene 1 (SLR1) is expressed in the papillar cells of Brassica stigmas from a few days before anthesis. We have previously shown that a 1500-bp fragment of the SLR1 gene promoter is sufficient to direct high-level, temporally regulated expression of the beta-glucuronidase reporter gene in the pistils of transgenic tobacco. We have carried out a deletion analysis of the SLR1 promoter and found that elements required for pistil expression are located between -258 and -327 bp (relative to the translation start site). Furthermore, specific binding of pistil nuclear factors to sequences within this region was demonstrated by gel retardation analysis. Sequences between -1350 and -1500 were found to be required for high-level expression.

摘要

甘蓝S位点相关基因1（SLR1）在花期前几天就在甘蓝柱头的乳头状细胞中表达。我们之前已经表明，SLR1基因启动子的一个1500bp片段足以指导β-葡萄糖醛酸酶报告基因在转基因烟草雌蕊中进行高水平的、受时间调控的表达。我们对SLR1启动子进行了缺失分析，发现雌蕊表达所需的元件位于-258至-327bp之间（相对于翻译起始位点）。此外，通过凝胶阻滞分析证明了雌蕊核因子与该区域内序列的特异性结合。发现-1350至-1500之间的序列是高水平表达所必需的。