Engel A M, Svane I M, Mouritsen S, Rygaard J, Clausen J, Werdelin O
Institute of Medical Microbiology and Immunology, University of Copenhagen, Panum Institute, Denmark.
APMIS. 1996 Sep;104(9):629-39. doi: 10.1111/j.1699-0463.1996.tb04923.x.
In order to study the role of the T-cell-mediated immune defense in tumor development, a total of 93 sarcomas were induced using different doses (8 micrograms (0.1%), 40 micrograms (0.5%) and 400 micrograms (5%)) of 3-methylcholanthrene in athymic nude Balb/c mice and phenotypically normal immunocompetent Balb/c mice. A shorter tumor induction time and a higher tumor incidence after treatment with low doses of methylcholanthrene were seen in nude mice than in immunocompetent mice, indicating that they have a lower resistance to the carcinogen. Contrary to expectations we found that the MHC class I expression of tumors from nude mice was lower than that of tumors from normal mice. Higher surface expression of MHC class I was demonstrated on high dose tumors from normal mice than on low dose tumors from normal mice. The cellular composition of the individual tumors raised in nude mice was more heterogeneous with respect to MHC class I expression. Since the mice differ genetically only with respect to the nu gene, these results indicate that a lack of T-cell-mediated defense mechanisms may confer upon the bearer a lower resistance to 3-methylcholanthrene and a different MHC profile of the ensuing tumor.
为了研究T细胞介导的免疫防御在肿瘤发生中的作用，分别用不同剂量（8微克（0.1%）、40微克（0.5%）和400微克（5%））的3-甲基胆蒽在无胸腺裸Balb/c小鼠和表型正常的免疫活性Balb/c小鼠中诱导出93个肉瘤。与免疫活性小鼠相比，裸鼠经低剂量甲基胆蒽处理后肿瘤诱导时间更短，肿瘤发生率更高，这表明它们对致癌物的抵抗力更低。与预期相反，我们发现裸鼠肿瘤的MHC I类表达低于正常小鼠肿瘤。正常小鼠高剂量肿瘤的MHC I类表面表达高于正常小鼠低剂量肿瘤。裸鼠中生长的单个肿瘤的细胞组成在MHC I类表达方面更加异质性。由于这些小鼠仅在nu基因方面存在遗传差异，这些结果表明缺乏T细胞介导的防御机制可能使宿主对3-甲基胆蒽的抵抗力降低，并使随后发生的肿瘤具有不同的MHC谱。
