亚甲蓝对大电导钙激活钾通道的激活作用。

Activation by methylene blue of large Ca(2+)-activated K+ channels.

作者信息

Stockand J D, Sansom S C

机构信息

Department of Medicine, University of Texas Medical School at Houston 77030, USA.

出版信息

Biochim Biophys Acta. 1996 Dec 4;1285(2):123-6. doi: 10.1016/s0005-2736(96)00194-0.

DOI:10.1016/s0005-2736(96)00194-0

PMID:8972695

Abstract

Using the patch-clamp method, we found that methylene blue (MB), a free radical inhibitor of guanylyl cyclase, activated large, Ca(2+)-activated K+ channels (BKCa) in either cell attached or excised, inside-out patches of human mesangial cells in culture. Since BKCa are important feedback regulators of contraction of smooth muscle and mesangial cells, these results indicate that MB may be an important opener of BKCa channels and a regulator of vascular volume and resistance.

摘要

运用膜片钳技术，我们发现，作为鸟苷酸环化酶的自由基抑制剂，亚甲蓝（MB）可激活培养的人系膜细胞在细胞贴附式或内面向外式膜片中的大电导、Ca(2+) 激活的钾通道（BKCa）。由于BKCa是平滑肌和系膜细胞收缩的重要反馈调节因子，这些结果表明，MB可能是BKCa通道的重要开放剂以及血管容量和阻力的调节因子。