Winston J L, Toth S I, Roe B A, Dyer D W
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, 14214, USA.
Gene. 1996 Nov 14;179(2):199-204. doi: 10.1016/s0378-1119(96)00317-4.
In a search for clones from a lambda gt11 expression library of Actinobacillus actinomycetemcomitans (Aa) genomic DNA that expressed epitopes from a 70-kDa iron-repressible membrane protein, we inadvertently identified clones that encoded a member of the 90-kDa heat-shock protein (HSP 90) family. The gene appears to encode a homologue of HtpG, as the nucleotide sequence has approximately 70% identity with the Escherichia coli (Ec) and Vibrio fischeri htpG. Growth of an Aa htpG insertion mutant at 42 degrees C was reduced to 50% of the parent strain, similar to an Ec htpG deletion mutant. These data suggest that Aa HtpG performs a function similar to Ec HtpG.
在从伴放线放线杆菌（Aa）基因组DNA的λgt11表达文库中寻找表达来自70 kDa铁抑制膜蛋白表位的克隆时，我们意外地鉴定出编码90 kDa热休克蛋白（HSP 90）家族成员的克隆。该基因似乎编码HtpG的同源物，因为核苷酸序列与大肠杆菌（Ec）和费氏弧菌的htpG具有约70%的同一性。Aa htpG插入突变体在42℃下的生长降至亲本菌株的50%，类似于Ec htpG缺失突变体。这些数据表明Aa HtpG执行与Ec HtpG相似的功能。
