[从各种植物物种中纯化过氧化物酶并分离同功过氧化物酶（作者译）]

[Purification of peroxidases and separation of isoperoxidases from various plant species (author's transl)].

作者信息

García-Barrenechea L M, Iglesias J L, Cabezas M, Cabezas J A

出版信息

Rev Esp Fisiol. 1977 Sep;33(3):233-8.

Abstract

The partial purification of peroxidase (EC.1.11.1.7) and separation of isoperoxidases by disc electrophoresis from Cucurbita Pepo L., Phaseolus vulgaris L., Cicer arietinum L. and Hordeum, Secale and Triticum sp., have been studied. Peroxidase from fruit of pumpkin and from 6-day-old coleoptiles of French bean and chick pea has been partially purified, 128-, 174-, and 140-fold, respectively. The apparent Km at the optimum pH were: pumpkin (epicarp.), 2.7 X 10(-4) M; barley, common rye and wheat (primary leaves, in all cases), 1.4 X 10(-5), 1.2 X 10(-5) and 3.1 X 10(-5) M, respectively. Isoperoxidases have been separated by disc electrophoresis on 7% polyacrylamide gel and stained with p-phenylenediamine. Differences in patterns of anodic and cathodic isozymes were observed: 3 isozymes from fruits of pumpkin, 4 from French bean, 4 from chick pea, 11 from leaves of barley, 10 from leaves of common rye and 9 from leaves of wheat.

摘要

对来自西葫芦、菜豆、鹰嘴豆以及大麦、黑麦和小麦的过氧化物酶（EC.1.11.1.7）进行了部分纯化，并通过圆盘电泳分离了同功过氧化物酶。南瓜果实、菜豆和鹰嘴豆6日龄胚芽鞘中的过氧化物酶已分别进行了128倍、174倍和140倍的部分纯化。在最适pH下的表观Km值分别为：南瓜（外果皮）2.7×10⁻⁴ M；大麦、普通黑麦和小麦（在所有情况下均为初生叶）分别为1.4×10⁻⁵、1.2×10⁻⁵和3.1×10⁻⁵ M。通过在7%聚丙烯酰胺凝胶上进行圆盘电泳并使用对苯二胺染色来分离同功过氧化物酶。观察到阳极和阴极同工酶模式存在差异：南瓜果实中有3种同工酶，菜豆中有4种，鹰嘴豆中有4种，大麦叶片中有11种，普通黑麦叶片中有10种，小麦叶片中有9种。