编码人转化生长因子-βⅡ型受体的cDNA的克隆：mRNA的异质性

Cloning of a cDNA encoding the human transforming growth factor-beta type II receptor: heterogeneity of the mRNA.

作者信息

Ogasa H, Noma T, Murata H, Kawai S, Nakazawa A

机构信息

Department of Biochemistry, Yamaguchi University School of Medicine, Japan.

出版信息

Gene. 1996 Nov 28;181(1-2):185-90. doi: 10.1016/s0378-1119(96)00501-x.

DOI:10.1016/s0378-1119(96)00501-x

PMID:8973329

Abstract

We have isolated two novel cDNAs encoding the transforming growth factor-beta (TGF-beta) type II receptor (TGF-beta IIR), termed TGF-beta IIR alpha and TGF-beta IIR beta 1 from a human fetal liver library. They have unique nucleotide (nt) sequences, compared with the reported TGF-beta IIR sequence, at the 5' end. Southern blot analysis using probes from each clone detected the specific genomic DNA fragments. RT-PCR analysis revealed a distinct pattern of expression for each isoform. These results indicated that TGF-beta IIR has heterogeneity in the structure, and the expression of TGF-beta IIR isoforms is differentially regulated. The heterogeneity of TGF-beta IIR molecules could be derived from alternative splicing and might elicit specific TGF-beta receptor functions.

摘要

我们从一个人胎儿肝脏文库中分离出了两个编码转化生长因子β（TGF-β）II型受体（TGF-β IIR）的新cDNA，分别称为TGF-β IIRα和TGF-β IIRβ1。与已报道的TGF-β IIR序列相比，它们在5'端具有独特的核苷酸（nt）序列。使用来自每个克隆的探针进行的Southern印迹分析检测到了特定的基因组DNA片段。RT-PCR分析揭示了每种异构体独特的表达模式。这些结果表明，TGF-β IIR在结构上具有异质性，并且TGF-β IIR异构体的表达受到差异调节。TGF-β IIR分子的异质性可能源自可变剪接，并可能引发特定的TGF-β受体功能。