转化生长因子α和β、表皮生长因子、角质形成细胞生长因子及其受体在胎儿和成人前列腺组织及癌细胞系中的差异基因表达。

Differential gene expression of transforming growth factors alpha and beta, epidermal growth factor, keratinocyte growth factor, and their receptors in fetal and adult human prostatic tissues and cancer cell lines.

作者信息

Dahiya R, Lee C, Haughney P C, Chui R, Ho R, Deng G

机构信息

Department of Urology, University of California San Francisco, USA.

出版信息

Urology. 1996 Dec;48(6):963-70. doi: 10.1016/s0090-4295(96)00376-7.

DOI:10.1016/s0090-4295(96)00376-7

PMID:8973690

Abstract

OBJECTIVES

Recent studies have shown that growth factors may play a role in the etiology of benign prostatic hyperplasia (BPH) and prostatic carcinoma. Several growth factors have been reported to be expressed by prostatic tissues, but these growth factors have never been examined in human fetal prostate and compared with adult prostates and cancer cell lines. The present study was designed to investigate the messenger ribonucleic acid (mRNA) expression of transforming growth factor (TGF)-alpha, TGF-beta 1, TGF-beta 2, TGF- beta 3, keratinocyte growth factor (KGF), epidermal growth factor (EGF), EGF receptor (EGF-R), and KGF receptor (KGF-R) in human fetal and adult prostatic tissues and cancer cell lines by reverse-transcriptase-polymerase chain reaction-(RT-PCR) using specific oligonucleotide primers.

METHODS

Total RNA was extracted from human fetal and adult prostates (BPH tissues) and cancer cell lines. The gene expression of these growth factors and their receptors was determined by RT-PCR using specific oligonucleotide primers.

RESULTS

The results of these experiments suggest that: (1) human fetal prostate expressed mRNA transcripts for TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, and EGF. However, KGF, KGF-R, and EGF-R mRNA were not expressed by human fetal prostate; (2) human adult prostate (BPH tissues) showed mRNA transcripts for all growth factors and their receptors except KGF-R; (3) human BPH-1 cell lines expressed mRNA transcripts for TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, EGF, and KGF-R, but not for EGF-R and KGF growth factors; (4) human primary prostate cancer cell line (ND-1) showed mRNA transcripts for all growth factors except EGF and KGF; and (5) human prostate cancer cell lines (LNCaP, DU-145, PC-3) expressed mRNA transcripts for all growth factors except KGF, which was absent in all cell lines. However, KGF-R mRNA was absent in the PC-3 prostate cancer cell line.

CONCLUSIONS

These results suggest that the differential gene expression for various growth factors and their receptors in human fetal and adult prostatic tissues and cancer cell lines may be important in understanding the role of these factors in the pathophysiology of prostatic diseases.

摘要

目的

近期研究表明，生长因子可能在良性前列腺增生（BPH）和前列腺癌的病因学中发挥作用。已有报道称几种生长因子在前列腺组织中表达，但这些生长因子从未在人胎儿前列腺中进行检测并与成人前列腺及癌细胞系进行比较。本研究旨在通过使用特异性寡核苷酸引物的逆转录 - 聚合酶链反应（RT-PCR），研究转化生长因子（TGF）-α、TGF-β1、TGF-β2、TGF-β3、角质形成细胞生长因子（KGF）、表皮生长因子（EGF）、EGF受体（EGF-R）和KGF受体（KGF-R）在人胎儿和成人前列腺组织及癌细胞系中的信使核糖核酸（mRNA）表达。

方法

从人胎儿和成人前列腺（BPH组织）及癌细胞系中提取总RNA。使用特异性寡核苷酸引物通过RT-PCR测定这些生长因子及其受体的基因表达。

结果

这些实验结果表明：（1）人胎儿前列腺表达TGF-α、TGF-β1、TGF-β2、TGF-β3和EGF的mRNA转录本。然而，人胎儿前列腺未表达KGF、KGF-R和EGF-R mRNA；（2）成人前列腺（BPH组织）除KGF-R外，所有生长因子及其受体均显示有mRNA转录本；（3）人BPH-1细胞系表达TGF-α、TGF-β1、TGF-β2、TGF-β3、EGF和KGF-R的mRNA转录本，但不表达EGF-R和KGF生长因子；（4）人原发性前列腺癌细胞系（ND-1）除EGF和KGF外，所有生长因子均显示有mRNA转录本；（5）人前列腺癌细胞系（LNCaP、DU-145、PC-3）除KGF外，所有生长因子均表达mRNA转录本，所有细胞系中均无KGF表达。然而，PC-3前列腺癌细胞系中无KGF-R mRNA。