Süss A, Reetz J, Schulze P, Kretzschmar M, Schirrmeister W, Süss J
Klinik für Anästhesiologie und Intensivmedizin, Klinikum der Stadt Gera.
Anaesthesiol Reanim. 1996;21(4):97-102.
Ornithosis is still a life-threatening illness unless diagnosed early and adequately treated with antibiotics. Diagnosis, however, is difficult because often no direct contact with birds is obvious. We report on the course of a severe case of ornithosis in a 45-year-old man who had no previous health problems. Infection with Chlamydia psittaci probably occurred by inhaling of contaminated dust produced by flocks of wild pigeons. A special feature of the case is that commonly used serological tests (complement fixation reaction, CFR; ELISA) failed to yield clear results. Since propagation of Chlamydia psittaci requires special biological laboratory containment facility no. 3, we were able to detect the pathogen in faeces, blood, and urine using the direct immunofluorescence antibody technique (DIFT) and a modified peroxidase antiperoxidase technique (PAP). A lot of Chlamydia psittaci was found. These results were verified by electron microscopy. As a result, the treatment could be quickly adjusted to the application of doxycyclin, which led to a rapid improvement in the patient's condition. This direct detection of Chlamydia psittaci by DIFT, the PAP technique or electron microscopy, also in faeces, blood and urine specimens, represents a useful addition to the usual microbiological methods for the diagnosis of this pathogen.
鹦鹉热仍然是一种危及生命的疾病，除非早期诊断并使用抗生素进行充分治疗。然而，诊断很困难，因为通常没有明显的与鸟类直接接触史。我们报告了一名45岁既往无健康问题的男性患严重鹦鹉热的病程。鹦鹉热衣原体感染可能是由于吸入野生鸽群产生的污染灰尘所致。该病例的一个特殊之处在于，常用的血清学检测（补体结合反应，CFR；酶联免疫吸附测定，ELISA）未能得出明确结果。由于鹦鹉热衣原体的繁殖需要特殊的生物实验室3级防护设施，我们能够使用直接免疫荧光抗体技术（DIFT）和改良的过氧化物酶抗过氧化物酶技术（PAP）在粪便、血液和尿液中检测到病原体。发现了大量鹦鹉热衣原体。这些结果通过电子显微镜得到了验证。因此，治疗能够迅速调整为应用强力霉素，这使患者的病情迅速好转。通过DIFT、PAP技术或电子显微镜在粪便、血液和尿液标本中直接检测鹦鹉热衣原体，是对该病原体诊断常用微生物学方法的有益补充。
