Jacob E, O'Brien H A, Mollin D L
Scand J Haematol. 1977 Aug;19(2):201-6. doi: 10.1111/j.1600-0609.1977.tb02345.x.
A new radioisotope dilution assay for vitamin B12-intrinsic factor complex is described. The method is based on the use of the binding type intrinsic factor antibody (the binding reagent), which when combined with the intrinsic factor-vitamin B12 complex (labelled ligand), is quantitatively adsorbed onto zirconium phosphate gel at pH 6.25. The new assay has been shown to provide a measure of intrinsic factor comparable with other intrinsic factor assays, but it has the important advantage of being able to measure the unlabelled vitamin B12-intrinsic factor complex (unlabelled ligand), and will, therefore, be valuable in the study of physiological events in the gastrointestinal tract. During the study, it was found that there is some evidence for a least two types of binding intrinsic factor antibody: One which combines preferentially with the intrinsic factor-vitamin B12 complex and one which combines equally well with this complex or with free intrinsic factor.
本文描述了一种用于维生素B12-内因子复合物的新型放射性同位素稀释测定法。该方法基于使用结合型内因子抗体（结合试剂），当它与内因子-维生素B12复合物（标记配体）结合时，在pH 6.25条件下会定量吸附到磷酸锆凝胶上。新测定法已被证明能够提供与其他内因子测定法相当的内因子测量值，但它具有能够测量未标记的维生素B12-内因子复合物（未标记配体）这一重要优势，因此在胃肠道生理事件的研究中将具有重要价值。在研究过程中，发现有证据表明至少存在两种类型的结合内因子抗体：一种优先与内因子-维生素B12复合物结合，另一种与该复合物或游离内因子结合的能力相当。
