Ammar D A, Eadie D M, Wong D J, Ma Y Y, Kolakowski L F, Yang-Feng T L, Thompson D A
Department of Ophthalmology, The University of Michigan Medical School, Ann Arbor, Michigan 48105, USA.
Genomics. 1996 Dec 15;38(3):392-8. doi: 10.1006/geno.1996.0642.
Neuropeptide Y (NPY) signals through a family of G-protein-coupled receptors present in the brain and sympathetic neurons. To further our understanding of the genetic elements involved in the regulation of NPY receptor expression, we have cloned and characterized the human gene encoding the type 2 NPY receptor (Y2 receptor, HGMW-approved symbol NPY2R).2 The transcript spans 9 kb of genomic sequence and is encoded on two exons. As in the type 1 NPY receptor (Y1 receptor) gene, the 5'-untranslated region of the Y2 receptor is interrupted by an intervening sequence ( approximately 4.5 kb). However, the Y2 receptor gene does not contain an intron analogous to that present in the coding region of the Y1 receptor. The predicted transcript size ( approximately 4.5 kb) is consistent with the size observed by Northern analysis. The 381-amino-acid sequence deduced from the open reading frame is identical to that encoded by the cDNA. The Y2 receptor gene maps to human chromosome 4q31, the same region containing the Y1 receptor locus, suggesting that these subtypes may have arisen by gene duplication despite their structural differences.
神经肽Y（NPY）通过存在于大脑和交感神经元中的一类G蛋白偶联受体发出信号。为了进一步了解参与NPY受体表达调控的遗传元件，我们克隆并鉴定了编码2型NPY受体（Y2受体，HGMW认可符号为NPY2R）的人类基因。该转录本跨越9kb的基因组序列，由两个外显子编码。与1型NPY受体（Y1受体）基因一样，Y2受体的5'非翻译区被一个间隔序列（约4.5kb）中断。然而，Y2受体基因不包含与Y1受体编码区中存在的内含子类似的内含子。预测的转录本大小（约4.5kb）与Northern分析观察到的大小一致。从开放阅读框推导的381个氨基酸序列与cDNA编码的序列相同。Y2受体基因定位于人类染色体4q31，该区域与Y1受体基因座相同，这表明尽管这些亚型存在结构差异，但可能是通过基因复制产生的。
