Deere M, Johnson J, Garza S, Harrison W R, Yoon S J, Elder F F, Kucherlapati R, Hook M, Hecht J T
Graduate School of Biological Sciences, The University of Texas Medical School, Houston 77225, USA.
Genomics. 1996 Dec 15;38(3):399-404. doi: 10.1006/geno.1996.0643.
PG-Lb is a small dermatan sulfate proteoglycan that has been previously characterized in chicken. In the developing limb, chick PG-Lb appears to be exclusively expressed in the zone of flattened chondrocytes. We have cloned and sequenced the human homolog to chick PG-Lb from two human chondrocyte cDNA libraries and a human chondrocyte RNA sample. The human homolog has been named DSPG3, as it is the third member of the small dermatan sulfate proteoglycan family to be identified and characterized along with biglycan (PG-I) and decorin (PG-II). DSPG3 maps to chromosome 12q21 and is composed of 1515 nucleotides of cDNA that code for a 322-amino-acid protein. The protein contains three potential glycosaminoglycan attachment sites, two N-glycosylation sites, a poly- glutamic acid stretch, and six cysteines. By Northern analysis, we have demonstrated that DSPG3 is expressed in cartilage, as well as ligament and placental tissues.
PG-Lb是一种小硫酸皮肤素蛋白聚糖，先前已在鸡身上得到表征。在发育中的肢体中，鸡PG-Lb似乎仅在扁平软骨细胞区表达。我们从两个人软骨细胞cDNA文库和一个人软骨细胞RNA样本中克隆并测序了鸡PG-Lb的人类同源物。该人类同源物被命名为DSPG3，因为它是小硫酸皮肤素蛋白聚糖家族中第三个被鉴定和表征的成员，与双糖链蛋白聚糖（PG-I）和核心蛋白聚糖（PG-II）一起。DSPG3定位于染色体12q21，由1515个核苷酸的cDNA组成，编码一个322个氨基酸的蛋白质。该蛋白质包含三个潜在的糖胺聚糖附着位点、两个N-糖基化位点、一个聚谷氨酸延伸段和六个半胱氨酸。通过Northern分析，我们证明DSPG3在软骨以及韧带和胎盘组织中表达。
