对多氯联苯长期生物降解实验评估产生负面影响的物理因素。

Physical factors negatively affecting evaluation of long-term biodegradation experiments of polychlorinated biphenyls.

作者信息

Zachar P, Novotný C, Voznáková Z, Matucha M, Tesarová E, Sýkora D, Kubátová A, Popl M, Sasek V

机构信息

Institute of Chemical Technology, Department of Analytical Chemistry, Prague, Czech Republic.

出版信息

Chemosphere. 1996 Dec;33(12):2411-21. doi: 10.1016/s0045-6535(96)00338-4.

DOI:10.1016/s0045-6535(96)00338-4

PMID:8976055

Abstract

Behaviour of PCB added in the form of a technical mixture (Delor 106) and [14C]PCB 77 into different aqueous model cultivation media in long-term experiments was studied. The work was focused on monitoring PCB recovery from liquid media in the absence of micro-organisms. GC-ECD and radioanalysis were utilised for determination of PCB in the examined systems. Strong relations between duration of experiments, composition of media, degree of chlorination of biphenyls, and recovery of PCB were found. The lowest yields of PCB were observed for the extracts from model solutions based on N-limited cultivation media without veratryl alcohol and Tween 80. The addition of these components, mainly of Tween 80, caused a significant increase of PCB recoveries.

摘要

在长期实验中，研究了以工业混合物（多氯联苯混合物106）和[14C]多氯联苯77形式添加到不同水性模型培养基中的多氯联苯的行为。这项工作重点是监测在没有微生物的情况下液体培养基中多氯联苯的回收率。气相色谱 - 电子捕获检测器（GC - ECD）和放射性分析用于测定受试系统中的多氯联苯。发现实验持续时间、培养基组成、联苯的氯化程度和多氯联苯回收率之间存在密切关系。对于基于不含藜芦醇和吐温80的氮限制培养基的模型溶液提取物，观察到多氯联苯的回收率最低。添加这些成分，主要是吐温80，导致多氯联苯回收率显著提高。