Hirasawa T, Yono K, Mikazuki K
Aburabi Laboratories, Shionogi Research Laboratories, Shiga, Japan.
Zentralbl Veterinarmed B. 1996 Nov;43(9):545-54. doi: 10.1111/j.1439-0450.1996.tb00352.x.
Prevalence of canine parvovirus type 2 (CPV-2) in Japanese dogs and genomic variations among the virus strains were examined. Two-step polymerase chain reaction with double-nested primer pairs designed in the NS and VP1/VP2 genes of CPV-2 was developed for the detection of the viral genome in faecal samples. A total of 74 samples obtained from diarrhoeal house dogs between 1993 and 1995 were tested by the PCR. The virus-positive rate was 54.1%, showing that CPV-2 is still involved in many cases of acute infectious diarrhoea in Japanese dogs. The VP1/VP2 gene of the positive samples was subjected to restriction fragment length polymorphism (RFLP) analysis and nucleotide sequencing. RFLP patterns of the samples were almost identical to those of one CPV-2 strain (TDKet-91-42) isolated in 1991, but different from those of the CPV-2 in the late 1970s and 1980s. The results suggest that a new genotype of CPV-2 appeared and spread among Japanese dogs in the early 1990s.
检测了日本犬中2型犬细小病毒（CPV - 2）的流行情况以及病毒株之间的基因组变异。开发了一种两步聚合酶链反应，使用针对CPV - 2的NS和VP1/VP2基因设计的双嵌套引物对，用于检测粪便样本中的病毒基因组。对1993年至1995年间从腹泻家养犬获得的74份样本进行了PCR检测。病毒阳性率为54.1%，表明CPV - 2仍与日本犬的许多急性感染性腹泻病例有关。对阳性样本的VP1/VP2基因进行了限制性片段长度多态性（RFLP）分析和核苷酸测序。样本的RFLP模式与1991年分离的一株CPV - 2毒株（TDKet - 91 - 42）几乎相同，但与20世纪70年代末和80年代的CPV - 2不同。结果表明，20世纪90年代初，CPV - 2的一种新基因型出现并在日本犬中传播。
