Department of Internal Medicine, Faculty of Veterinary Medicine, Harran University, Sanliurfa.
Onderstepoort J Vet Res. 2019 Aug 27;86(1):e1-e8. doi: 10.4102/ojvr.v86i1.1734.
Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey's dog population.
犬细小病毒-2（CPV-2）是一种感染犬的传染性病毒病的病原体，其特征为腹泻和呕吐。CPV-2 基因组的突变产生了在全球范围内传播的新变体。本文报告了在土耳其东南部安纳托利亚的犬群中收集的 CPV-2 变体的分子分析。为了对疑似细小病毒的犬进行实验室诊断，先前采集了 20 份血液样本，通过聚合酶链反应（PCR）对 CPV-2 进行了筛选。在 20 个样本中，有 18 个样本对 CPV-2 呈阳性。部分 VP2 基因测序和限制性片段长度多态性（RFLP）分析显示 CPV-2a（n = 1）、CPV-2b（n = 16）和 CPV-2c（n = 1）变体。基于部分长度 VP2 基因的系统进化分析显示，CPV-2b（n = 15）变体在系统进化树上单独聚类。CPV-2c 样本与中国株和印度尼西亚株在系统进化上相关，而 CPV-2a 样本与葡萄牙株在系统进化上相关。这些结果首次证明 CPV-2c 存在于土耳其东南部安纳托利亚的犬群中，表明 CPV-2a/2b/2c 变体在土耳其的犬群中共同存在。
