Bessler H, Straussberg R, Gurary N, Aloni D, Sirota L
Department of Pediatrics, Golda Medical Center, Hasharon Hospital, Petah-Tiqva, Israel.
Arch Dis Child Fetal Neonatal Ed. 1996 Nov;75(3):F197-201. doi: 10.1136/fn.75.3.f197.
The effect of dexamethasone on interleukin 2 (IL-2) and interleukin 3 (IL-3) production by mononuclear cells in preterm and term infants and adults was evaluated. The capacity of mononuclear cells to produce these cytokines, in preterm infants with bronchopulmonary dysplasia (BPD) and treated with dexamethasone, was compared with that before treatment. Twenty six preterm and 36 term neonates and 24 healthy adults were included in the study. Mononuclear cells isolated from neonatal cord blood (CBMC) and adult peripheral blood (PBMC) were stimulated with phytohaemagglutinin (PHA) in the absence or presence of dexamethasone at concentrations between 10(-8)M and 10(-5)M. IL-2 and IL-3 activities in the supernatant fluids were tested using bioassays. The in vivo effect of the drug on the production of these cytokines by PBMC in 10 preterms was determined before and 24 hours after dexamethasone administration (0.5 mg/kg/day). The production of both cytokines was inhibited in a dose dependent manner. A difference in the sensitivity of mononuclear cells to the inhibitory effect of the drug was found between neonatal cord blood cells and adult PBMC, the former being more sensitive. PBMC from preterm infants treated with dexamethasone for BPD produced significantly less IL-2 and IL-3 as early as 24 hours after the initiation of the treatment (43% and 31%; P < 0.05, respectively). It is concluded that mononuclear cells from preterm and term neonates are more sensitive to the inhibitory effect of dexamethasone on IL-2 and IL-3 production.
评估了地塞米松对早产和足月婴儿及成人单核细胞产生白细胞介素2（IL - 2）和白细胞介素3（IL - 3）的影响。将患有支气管肺发育不良（BPD）并接受地塞米松治疗的早产婴儿单核细胞产生这些细胞因子的能力与治疗前进行了比较。该研究纳入了26名早产新生儿、36名足月新生儿和24名健康成人。从新生儿脐带血（CBMC）和成人外周血（PBMC）中分离出的单核细胞在不存在或存在浓度介于10^(-8)M至10^(-5)M之间的地塞米松的情况下，用植物血凝素（PHA）进行刺激。使用生物测定法检测上清液中的IL - 2和IL - 3活性。在10名早产儿中，在给予地塞米松（0.5mg/kg/天）之前和之后24小时，测定了该药物对PBMC产生这些细胞因子的体内作用。两种细胞因子的产生均呈剂量依赖性抑制。发现新生儿脐带血细胞与成人PBMC之间单核细胞对药物抑制作用的敏感性存在差异，前者更敏感。因BPD接受地塞米松治疗的早产婴儿的PBMC在治疗开始后24小时就显著减少产生IL - 2和IL - 3（分别为43%和31%；P < 0.05）。结论是早产和足月新生儿的单核细胞对地塞米松对IL - 2和IL - 3产生的抑制作用更敏感。
