Meng F J, Giwercman A, Skakkebaek N E
University Department of Growth and Reproduction, Rigshospitalet, Copenhagen, Denmark.
J Pathol. 1996 Oct;180(2):206-13. doi: 10.1002/(SICI)1096-9896(199610)180:2<206::AID-PATH640>3.0.CO;2-Y.
The silver staining which specifically stains argyrophilic proteins (AgNORs) in interphase nuclei was applied to paraffin sections of 24 testicular specimens with carcinoma in situ (CIS). AgNOR area per nucleus was quantified by a computerized image analyser. Significant quantitative differences were found between CIS, Sertoli cells, and spermatogonia (P = 0.0001), with median values of 10.3, 2.8, and 1.4 microns2 in the three cell types, respectively. A Sertoli cell index (SCI), defined as the ratio between AgNORs in CIS or spermatogonia and Sertoli cells, was shown to be significant in the differential diagnosis of CIS cells from spermatogonia when 1.0 was used as the cut-off value (CIS > 1; spermatogonia < 1). Furthermore, CIS associated with non-seminoma was found to have a significantly higher level of AgNORs than CIS associated with pure seminoma (P < 0.01), indicating that subclonal variation in transformation potential might be present within morphologically identical CIS of the testis. It remains to be seen whether quantification of AgNORs in isolated CIS could be used to predict transformation of CIS into seminoma or non-seminoma.
将专门用于对间期细胞核中嗜银蛋白（AgNORs）进行染色的银染法应用于24例原位癌（CIS）睾丸标本的石蜡切片。通过计算机图像分析仪对每个细胞核的AgNOR面积进行定量分析。结果发现，CIS、支持细胞和精原细胞之间存在显著的定量差异（P = 0.0001），这三种细胞类型的中位数分别为10.3、2.8和1.4平方微米。支持细胞指数（SCI）定义为CIS或精原细胞与支持细胞中AgNORs的比值，当以1.0作为临界值时（CIS > 1；精原细胞 < 1），SCI在鉴别CIS细胞与精原细胞方面具有显著意义。此外，发现与非精原细胞瘤相关的CIS的AgNORs水平显著高于与纯精原细胞瘤相关的CIS（P < 0.01），这表明在睾丸形态学相同的CIS中可能存在转化潜能的亚克隆变异。孤立CIS中AgNORs的定量分析能否用于预测CIS向精原细胞瘤或非精原细胞瘤的转化还有待观察。
