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[通过负染、超薄切片及扫描电子显微镜对肺炎支原体的亚微观结构研究]

[Submicroscopic organization of M. pneumoniae studied by means of negative contrasting, ultrathin sections and scanning electron microscopy].

作者信息

Konstantinova N D, Barkhatova O I, Baĭzhomartov M S

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1979 Jul(7):66-71.

PMID:89769
Abstract

The ultrastructure of M. pneumoniae, grown on a solid culture medium and in a liquid one, was studied by a number of methods. Two types of cells were shown to prevail in the culture: spherical cells (0.5--1 micrometer) forming chains of different configurations and filamentous cells (5 micrometer long and greater) with spherical enlargements along their whole length. The absence of microcapsules made M. pneumoniae different from other species of mycoplasms, and the organism proliferated by division into 2 daughter cells, equal or unequal in size, by the segmentation of the cytoplasm and the formation of elementary bodies inside the cell and on its surface.

摘要

通过多种方法研究了在固体培养基和液体培养基上生长的肺炎支原体的超微结构。结果显示培养物中主要存在两种类型的细胞:球形细胞(0.5 - 1微米)形成不同构型的链,以及丝状细胞(长度为5微米及更长),其全长有球形膨大。缺乏微荚膜使肺炎支原体不同于其他支原体物种,该生物体通过细胞质分割以及在细胞内和细胞表面形成原体,分裂为两个大小相等或不等的子细胞进行增殖。

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