Pasquinelli G, Scala C, Borsetti G P, Martegani F, Laschi R
Scan Electron Microsc. 1985(Pt 3):1133-42.
In order to obtain useful and complete information on the study of pathological material, we observed by scanning electron microscopy (SEM) the same semithin sections observed by light microscopy (LM). For this purpose, the specimen must have, at the same time, chromatic and electron dense characteristics. We thus developed different specimen preparation methods, subjecting the semithin sections to specific polychromatic staining with high atomic number (Z) elements, to monochromatic staining followed by routine contrasting with uranyl acetate and lead citrate, and to specific cytochemical and immunocytochemical procedures. The specimens were examined in sequence by LM, by SEM equipped with secondary electron, backscattered electron, transmitted electron detectors and by scanning transmission electron microscopy (S(T)EM).
为了获取有关病理材料研究的有用且完整的信息，我们通过扫描电子显微镜（SEM）观察了用光镜（LM）观察过的相同半薄切片。为此，标本必须同时具备显色和电子致密特性。因此，我们开发了不同的标本制备方法，使半薄切片用高原子序数（Z）元素进行特定的多色染色，进行单色染色后再用醋酸铀和柠檬酸铅进行常规对比，以及进行特定的细胞化学和免疫细胞化学程序。标本依次通过光镜、配备二次电子、背散射电子、透射电子探测器的扫描电子显微镜以及扫描透射电子显微镜（S(T)EM）进行检查。
