Barbrook A C, Packer J C, Howe C J
Department of Biochemistry and Cambridge Centre for Molecular Recognition, University of Cambridge, UK.
FEBS Lett. 1996 Dec 2;398(2-3):198-200. doi: 10.1016/s0014-5793(96)01239-2.
Proteins targeted to the thylakoid lumen of plants and cyanobacteria and the periplasmic space of cyanobacteria are synthesised with N-terminal presequences which are removed following translocation across the membrane. These presequences are thought to direct translocation of the preprotein by a sec-type pathway. Detergent extracts of cyanobacterial and chloroplast membranes contain enzymes which are capable of processing precursors to the mature size. We show that the processing of a range of precursors by both cyanobacterial and chloroplast enzymes is inhibited by the penem SB216357. This is the first report of an inhibitor of these enzymes and indicates that they are type 1 signal peptidases.
靶向植物和蓝细菌类囊体腔以及蓝细菌周质空间的蛋白质是通过N端前序列合成的，这些前序列在跨膜转运后被去除。这些前序列被认为通过sec型途径指导前体蛋白的转运。蓝细菌和叶绿体膜的去污剂提取物含有能够将前体加工成成熟大小的酶。我们发现，青霉烯类化合物SB216357可抑制蓝细菌和叶绿体酶对一系列前体的加工。这是关于这些酶抑制剂的首次报道，表明它们是1型信号肽酶。
