Guo W X, Nichol M, Merlie J P
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, St. Louis, MO 63110, USA.
FEBS Lett. 1996 Dec 2;398(2-3):259-64. doi: 10.1016/s0014-5793(96)01216-1.
We have cloned and sequenced mouse utrophin cDNA, and successfully expressed full length utrophin (400 kDa) in both muscle and non-muscle cells. The expression of recombinant utrophin is compared with that of its homologue, dystrophin (427 kDa). We demonstrate that recombinant utrophin is targeted into agrin-induced acetylcholine receptor (AChR) clusters, while recombinant dystrophin is evenly distributed along cell membranes in cultured Sol 8 muscle cells. This observation suggests that utrophin and dystrophin may interact with different cytoskeletal proteins. The C-terminal domains are found to be responsible for the association of utrophin with AChR clusters.
我们已经克隆并测序了小鼠抗肌萎缩蛋白聚糖（utrophin）的互补脱氧核糖核酸（cDNA），并成功在肌肉细胞和非肌肉细胞中表达了全长抗肌萎缩蛋白聚糖（400千道尔顿）。将重组抗肌萎缩蛋白聚糖的表达与其同源物肌营养不良蛋白（dystrophin，427千道尔顿）的表达进行了比较。我们证明，重组抗肌萎缩蛋白聚糖靶向聚集到聚集蛋白诱导的乙酰胆碱受体（AChR）簇中，而重组肌营养不良蛋白在培养的Sol 8肌肉细胞中沿细胞膜均匀分布。这一观察结果表明，抗肌萎缩蛋白聚糖和肌营养不良蛋白可能与不同的细胞骨架蛋白相互作用。发现C末端结构域负责抗肌萎缩蛋白聚糖与AChR簇的结合。
