St Swierzko A, Shashkov A S, Senchenkova S N, Toukach F V, Ziolkowski A, Cedzynski M, Paramonov N A, Kaca W, Knirel Y A
Center of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland.
FEBS Lett. 1996 Dec 2;398(2-3):297-302. doi: 10.1016/s0014-5793(96)01061-7.
An acidic O-specific polysaccharide from the lipopolysaccharide of Proteus mirabilis O10 contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, the last-named sugar having not been found hitherto in O-antigens. Structure of a branched tetrasaccharide repeating unit of the polysaccharide was established by 1H and 13C NMR spectroscopy, including two-dimensional COSY and rotating-frame NOE spectroscopy. The lateral L-altruronic acid residue plays the immunodominant role in manifestation of the O10 specificity of Proteus, whereas a disaccharide fragment of the main chain in common with the O-specific polysaccharide of P. mirabilis O43 provides the one-way serological cross-reactivity between anti-O10 serum and O43-antigen.
