Susaki Y, Shimizu S, Katakura K, Watanabe N, Kawamoto K, Matsumoto M, Tsudzuki M, Furusaka T, Kitamura Y, Matsuda H
Department of Veterinary Clinic, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Japan.
Blood. 1996 Dec 15;88(12):4630-7.
The stimulating effect of nerve growth factor (NGF) on phagocytosis, parasite killing, and interleukin-1beta (IL-1beta) production of murine peritoneal macrophages was assessed. In the presence of various doses of NGF, macrophages showed the increased phagocytosis of both nonspecific hydrophilic microspheres and sheep red blood cells (SRBC) opsonized with anti-SRBC antibodies (Ab) or complement in a dose-dependent manner. NGF also enhanced killing of Leishmania donovani promastigotes by macrophages, and its ability was comparable with that of an optimal dose of recombinant granulocyte-macrophage colony-stimulating factor or recombinant interferon-gamma. The addition of NGF to peritoneal macrophages and monocyte-macrophage J774A.1 cells led to a significant release of IL-1beta in a dose-dependent manner and expression of IL-1beta mRNA. Because pretreatment of peritoneal macrophages and J774A.1 cells with K-252a, a tyrosine kinase inhibitor, completely suppressed these NGF-mediated stimulating effects and p140trk phosphorylation and because flow cytometric analysis with specific Ab against two distinct NGF receptors showed the expression of p140trk, unlike p75LNGFR, on the surface of macrophages, the stimulating activity of NGF to murine macrophages may be mediated through p140trk. Thus, NGF may act as an activator for murine macrophages in the process of inflammatory and immune actions.
评估了神经生长因子（NGF）对小鼠腹腔巨噬细胞吞噬作用、杀灭寄生虫以及白细胞介素-1β（IL-1β）产生的刺激作用。在存在不同剂量NGF的情况下，巨噬细胞对非特异性亲水性微球以及用抗绵羊红细胞（SRBC）抗体（Ab）或补体调理的绵羊红细胞（SRBC）的吞噬作用呈剂量依赖性增加。NGF还增强了巨噬细胞对杜氏利什曼原虫前鞭毛体的杀伤作用，其能力与最佳剂量的重组粒细胞-巨噬细胞集落刺激因子或重组干扰素-γ相当。向腹腔巨噬细胞和单核巨噬细胞J774A.1细胞中添加NGF导致IL-1β以剂量依赖性方式显著释放并表达IL-1β mRNA。由于用酪氨酸激酶抑制剂K-252a预处理腹腔巨噬细胞和J774A.1细胞可完全抑制这些NGF介导的刺激作用和p140trk磷酸化，并且由于用针对两种不同NGF受体的特异性抗体进行的流式细胞术分析显示巨噬细胞表面表达p140trk，与p75LNGFR不同，NGF对小鼠巨噬细胞的刺激活性可能是通过p140trk介导的。因此，NGF在炎症和免疫作用过程中可能作为小鼠巨噬细胞的激活剂。
