Nielsen P J, Georgiev O, Lorenz B, Schaffner W
Max Planck Institute for Immunobiology, Freiburg, Germany.
Eur J Immunol. 1996 Dec;26(12):3214-8. doi: 10.1002/eji.1830261255.
The protein Bob1 (also called OCA-B or OBF-1) is a B lymphocyte-specific transcriptional co-activator which can stimulate transcription by interaction with Oct-1 or Oct-2 and the octamer sequence found in the promoter and most enhancer regions of the immunoglobulin genes. The role that Bob1 plays in the maturation and function of B cells was investigated in mice deficient for Bob1. Although early stages of B cell development in the bone marrow do not appear to be affected, these mice show reduced numbers of B cells in peripheral lymphoid organs. Based on staining for B220, heat-stable antigen, and IgD, this reduction is particularly strong in those cells representing the more mature B cell stages. Probably as a consequence of this reduction in mature B cells, Bob1-deficient mice show reduced serum titers of the immunoglobulin isotypes IgG1, IgG2a, IgG2b and IgA, but not IgM. Histological examination of sections from spleen and lymph nodes reveal that while Bob-1-deficient mice have primary follicles, they lack well-developed germinal centers. Interestingly, B1 (Ly1) B cells in the peritoneum do not appear to be affected by the lack of Bob1. Taken together, these results suggest that, at least in conventional B cells, Bob1 plays in important role in the antigen-driven stages of B cell activation and maturation.
蛋白质Bob1（也称为OCA - B或OBF - 1）是一种B淋巴细胞特异性转录共激活因子，它可以通过与Oct - 1或Oct - 2以及在免疫球蛋白基因启动子和大多数增强子区域中发现的八聚体序列相互作用来刺激转录。在缺乏Bob1的小鼠中研究了Bob1在B细胞成熟和功能中的作用。虽然骨髓中B细胞发育的早期阶段似乎未受影响，但这些小鼠外周淋巴器官中的B细胞数量减少。基于对B220、热稳定抗原和IgD的染色，这种减少在代表更成熟B细胞阶段的细胞中尤为明显。可能由于成熟B细胞数量减少，缺乏Bob1的小鼠血清中免疫球蛋白同种型IgG1、IgG2a、IgG2b和IgA的滴度降低，但IgM未受影响。对脾脏和淋巴结切片的组织学检查表明，虽然缺乏Bob - 1的小鼠有初级滤泡，但它们缺乏发育良好的生发中心。有趣的是，腹膜中的B1（Ly1）B细胞似乎不受Bob1缺乏的影响。综上所述，这些结果表明，至少在传统B细胞中，Bob1在B细胞激活和成熟的抗原驱动阶段发挥重要作用。
