Rezzani R, Rodella L, Milanesi S, Bianchi R
Department of Biomedical Sciences and Biotechnology, University of Brescia, Italy.
Int J Exp Pathol. 1996 Oct;77(5):191-6. doi: 10.1046/j.1365-2613.1996.9790317.x.
In the present study, a set of two monoclonal antibodies (TRPM1, TRPM2) was used to investigate the macrophage populations in the rat thymus and their different sensitivities to cyclosporine-A (CsA). With double immunohistochemical staining we demonstrated that, in the normal rat thymus, there are 3 populations of macrophages (TRPM1+, TRPM1/2+, TRPM2+), present in different proportions throughout the thymus. In the outer cortex TRPM1+ and TRPM1/2+ were present, but the TRPM1/2+ cells were more numerous. No TRPM2+ cells were observed in this area. The cortex and medulla showed all 3 types of cells with a majority of TRPM1/2+ cells. In the corticomedullary zone (CMZ) TRPM1/2+ and TRPM2+ macrophages were present in about equal proportion while only a few TRPM1+ cells were observed. After CsA treatment (for 21 days) profound changes occurred in the thymus; we observed a complete disappearance of the thymic medulla and a reduction in the total number of macrophages. The TRPM1+ macrophages had been eliminated, a few TRPM1/2+ cells were found while many of the cells were TRPM2+. The presence of the macrophages in different thymic areas suggests that they are a very heterogeneous population. The possible significance of the macrophage heterogeneity and the relationship to CsA sensitivity is discussed.
在本研究中，使用一组两种单克隆抗体（TRPM1、TRPM2）来研究大鼠胸腺中的巨噬细胞群体及其对环孢素A（CsA）的不同敏感性。通过双重免疫组织化学染色，我们证明，在正常大鼠胸腺中，存在3种巨噬细胞群体（TRPM1+、TRPM1/2+、TRPM2+），在整个胸腺中以不同比例存在。在皮质外层存在TRPM1+和TRPM1/2+细胞，但TRPM1/2+细胞数量更多。在该区域未观察到TRPM2+细胞。皮质和髓质显示出所有3种类型的细胞，其中大多数为TRPM1/2+细胞。在皮质髓质交界区（CMZ），TRPM1/2+和TRPM2+巨噬细胞的比例大致相等，而仅观察到少数TRPM1+细胞。CsA处理（21天）后，胸腺发生了深刻变化；我们观察到胸腺髓质完全消失，巨噬细胞总数减少。TRPM1+巨噬细胞已被清除，发现少数TRPM1/2+细胞，而许多细胞为TRPM2+。巨噬细胞在胸腺不同区域的存在表明它们是一个非常异质的群体。讨论了巨噬细胞异质性的可能意义及其与CsA敏感性的关系。
