Ogura A, Noguchi Y, Yamamoto Y, Shibata S, Asano T, Okamoto Y, Honda M
Department of Veterinary Science, National Institute of Health, Tokyo, Japan.
Int J Exp Pathol. 1996 Oct;77(5):201-6. doi: 10.1046/j.1365-2613.1996.9830321.x.
Human immunodeficiency virus type 1 (HIV-1) was immunohistochemically and ultrastructurally localized in human thymus implants in SCID-hu mice 3 weeks after intravenous (i.v.) inoculation of the virus. A viral antigen (gp120) was predominantly distributed in and around the epithelial cells in Hassall's corpuscles as demonstrated by fluorescence immunohistochemistry. Occasional solitary round cells positive for the viral antigen but negative for cytokeratin were detected in the perivascular areas. Ultrastructural examinations clearly revealed a number of mature viral particles in the intercellular spaces of the Hassall's corpuscles. Thus the present study indicates the possibility that thymic epithelial cells in Hassall's corpuscles act as a target and/or reservoir in an early stage of HIV infection.
静脉注射1型人类免疫缺陷病毒（HIV-1）3周后，通过免疫组织化学和超微结构方法在SCID-hu小鼠的人胸腺植入物中定位到了该病毒。荧光免疫组织化学显示，病毒抗原（gp120）主要分布在哈氏小体的上皮细胞内及其周围。在血管周围区域偶尔检测到病毒抗原呈阳性但细胞角蛋白呈阴性的单个圆形细胞。超微结构检查清楚地显示在哈氏小体的细胞间隙中有许多成熟的病毒颗粒。因此，本研究表明哈氏小体中的胸腺上皮细胞在HIV感染早期可能作为靶细胞和/或病毒储存库。
