Iordache A, Bayle J, Chambon P
Laboratoire de Biochimie, Hôpital Pasteur, Nice, France.
Pathol Biol (Paris). 1996 Oct;44(8):720-3.
The authors described an assay procedure for the determination of free and total carnitine in red blood cells. This method is based on ultrafiltration of the broken red blood cells in Centrisart filter, by centrifugation. Free and total carnitine in ultrafiltrates is determined by the spectrophotometric method of Cederblad and Coll., adapted to Cobas-Fara. They evaluated the procedure linearity, reproductability and analytical recovery. Comparison with precipitate protein procedure showed that results correlated well. The advantages of this original assay include simplicity, increase rapidity, lower cost of reagents and mainly improved reproductability.
作者描述了一种用于测定红细胞中游离肉碱和总肉碱的检测方法。该方法基于在Centrisart过滤器中对破碎的红细胞进行超滤，通过离心实现。超滤液中的游离肉碱和总肉碱采用Cederblad等人的分光光度法测定，并适配于Cobas - Fara仪器。他们评估了该方法的线性、重复性和分析回收率。与沉淀蛋白法的比较表明结果相关性良好。这种原创检测方法的优点包括操作简单、速度加快、试剂成本降低，主要是重复性得到了提高。
