Asahi M, Hoshimaru M, Uemura Y, Tokime T, Kojima M, Ohtsuka T, Matsuura N, Aoki T, Shibahara K, Kikuchi H
Department of Neurosurgery, Faculty of Medicine, Kyoto University, Japan.
J Cereb Blood Flow Metab. 1997 Jan;17(1):11-8. doi: 10.1097/00004647-199701000-00003.
Recent investigations have been suggesting that some neuronal subpopulations may die via programmed cell death after focal ischemic injury. To clarify the possible roles of the genes involved in the cell-death program, this study examined the expression of three members of the interleukin-1 beta converting enzyme (Ice) gene family (Ice, Nedd2, and Yama/CPP32) and two members of the bcl-2 gene family (bcl-2 and bcl-x) in the rat brain after permanent occlusion of the middle cerebral artery. Northern blot analysis revealed a transient induction of Nedd2 mRNA 8 h after the ischemic insult (3.8-fold) and an increase in Yama/CPP32 mRNA 16 to 24 h after the insult (5.8-fold at 24 h), whereas the expression of Ice remained constant. The expression of bcl-2 and bcl-x remained constant after the ischemic insult. Taking into account the key role of the Ice gene family in the execution of programmed cell death, the induction of Ice gene family might play a causative role in apoptotic cell death.
最近的研究表明，局灶性缺血性损伤后，一些神经元亚群可能通过程序性细胞死亡而死亡。为了阐明参与细胞死亡程序的基因的可能作用，本研究检测了大脑中动脉永久性闭塞后大鼠脑内白细胞介素-1β转化酶（Ice）基因家族的三个成员（Ice、Nedd2和Yama/CPP32）以及bcl-2基因家族的两个成员（bcl-2和bcl-x）的表达。Northern印迹分析显示，缺血损伤后8小时Nedd2 mRNA出现短暂诱导（3.8倍），损伤后16至24小时Yama/CPP32 mRNA增加（24小时时为5.8倍），而Ice的表达保持恒定。缺血损伤后bcl-2和bcl-x的表达保持恒定。考虑到Ice基因家族在程序性细胞死亡执行中的关键作用，Ice基因家族的诱导可能在凋亡性细胞死亡中起因果作用。
